HIF-1α介导BMSCs复合PLGA修复大鼠颅骨标准骨缺损的研究

摘要

目的 构建低氧诱导因子(HIF)-1α介导骨髓间充质干细胞(BMSCs)复合聚乳酸-聚乙醇酸共聚物(PLGA)支架材料血管化组织工程骨,观察其在大鼠颅骨标准骨缺损中的修复效果.方法 将目的基因HIF-1 α转染至BMSCs后,与支架材料PLGA复合,修复SD大鼠颅骨双侧直径5 mm的标准骨缺损(n=18),随机分为3组:实验组植入HIF-1 α-BM-SCs/PLGA复合体(n=6),对照组植入BMSCs/PLGA复合体(n=6),材料组仅植入PLGA支架材料(n=6).术后8周处死大鼠取材,分别行大体观察、X线片检查和HE染色观察缺损区骨形成情况.结果 大体观察、X线片检查和HE染色均显示实验组缺损区新骨形成量明显大于对照组及材料组,差异有统计学意义(P＜0.05).结论 体内研究表明HIF-1α能够介导BMSCs促进骨组织形成,PLGA是理想的支架材料,用其构建的血管化工程骨能够有效修复骨缺损.%Objective To construct the vascular tissue engineering bone of hypoxia-inducible factor-1 α (HIF-1α)mediated bone mesenchymal stem cells (BMSCs) combined with polylactic-co-glycolicacid (PLGA) scaffold,and to observe the effect on repairing the critical-sizedcalvarial bone defect in rat.Methods The target gene HIF-1 α was transfected into BMSCs,and combined with PLGA scaffold,then implanted to repair bilateralcalvarial defect (Φ 5 mm) of SD rat (n =18).The rats were randomly divided into three groups:HIF-1α-BMSCs/PLGA group (n =6),BMSCs/PLGA group (n =6) and only PLGA scaffold group (n =6).The new bone formation was observed by gross inspection,X-ray examination and HE staining respectively at 8 weeks after surgery.Results The gross inspection,X-ray examination and HE staining showed that the amount of new bone formation in the experimental group was significantly higher than that in the control group and the material group,and the difference was statistically significant (P ＜ 0.05).Conclusion HIF-1 can mediate BMSCs to promote bone tissue formation.PL-GA is an ideal scaffold material and the bone defect can be repaired effectively by using this vascular tissue engineering bone.