miR-105-1在原发性肝细胞癌中表达的研究

摘要

目的 定量分析miRNA-105-1(miR-105-1)在原发性肝细胞癌(HCC)及癌旁正常组织中表达水平的差异.方法 取患者手术新鲜组织标本(10对T-N配对组织和另外40例原发性肝癌组织)提取RNA,采用实时荧光定量PCR(RT-qPCR) 技术检测标本中miR-105-1基因表达量.并下载公共基因表达数据库(GEO)芯片数据加以验证,通过2-ΔΔCt方法计算得到.结果 实验结果表明在10对配对的HCC组织中的miR-105-1的表达量显著低于癌旁正常组织,差异有统计学意义(P=0.046).在50个HCC组织的表达量显著低于10个癌旁组织,差异有统计学意义(P=0.031).GEO数据库芯片GSE22058-GPL10457中包含96个配对的HCC与癌旁正常组织,miR-105-1在HCC中下调,差异有统计学意义(P=0.035).GSE21362中包含73个配对的HCC与癌旁正常组织,miR-105-1在HCC中也是下调的,差异有统计学意义(P=0.042).结论 miR-105-1在原发性肝癌患者中较正常人群表达量低,可能是HCC患者发生发展的重要分子之一,并可以作为判断预后的一个指标和治疗的一个新靶点.%Objective To explore the expression level of miR-105-1 in hepatocellular carcinoma(HCC).Methods Total RNA was extracted from 10 pairs Tumor-Non-tumor(T-N) pairing tissue samples and another 40 HCC tissue samples, real-time quantitative PCR was performed.The 2-ΔΔCt method was used to quantify the expression level of miR-105-1.The gene chip data of HCC obtained from the GEO database were used to analyze the expression level of miR-105-1 in liver cancer.Results The results showed that the expression of HCC tissues of miR-105-1 was significantly lower than adjacent normal tissues in the 10 T-N paired tissue samples, the difference was statistically significant between the two(P=0.046).Equally, the expression of HCC tissues of miR-105-1 was significantly lower in the 50 HCC tissue samples than that in 10 adjacent normal tissues, the difference was statistically significant(P=0.031).The gene chip from GEO database(GSE22058-GPL10457) including 96 T-N paired HCC tissue samples suggested that miR-105-1 was down-regulated(P=0.035).Another data(GSE21362) including 73 T-N paired HCC tissue samples indicated that miR-105-1 was also down-regulated (P=0.042).Conclusion miR-105-1 is an important molecule in the progress of HCC patients.The expression level of HCC patients is lower than healthy people.It might be used as a prognostic indicator and a new target for HCC therapy.