栽培烟草抗逆相关WRKY转录因子的表达特征分析

摘要

采用RT-PCR反应从栽培烟草中克隆获得三条全长WRKY家族保守序列，长度分别为677bp、672bp、629bp,定名为NtWRKY10、NtWRKY11、NtWRKY12。生物信息分析表明三个基因与已经报道的拟南芥WRKY家族基因具有较高同源性，保守结构域属于第二类WRKY转录因子。RT-PCR分析表明，SA、ABA、MeJA等信号分子均能诱导这三个基因的上调或下调表达，表明这三个基因可能在栽培烟草抗逆反应中起作用。%Three full-length WRKY-domain cDNA sequence of 677bp, 672bp and 629bp, named NtWRKY10, NtWRKY11 and NtWRKY12 were cloned by RT-PCR from cultivated tobacco induced by SA, ABA and ET. Bioinformatics analysis showed that the three genes were highly similar with the reported WRKY family genes in Arabidopsis thaliana, which belonged to the second WRKY transcription. Results showed that molecules signals such as ABA, SA, MeJA could induce up-regulated expression or dowm-regulated expression, which indicated that the three genes play a role in stress response in cultivated tobacco.