一个与烟草TMV抗性基因N紧密连锁的共显性SSR标记

摘要

烟草普通花叶病(Tobacco MosaicVirus,TMV)是烟叶生产的主要病害,为建立烟草抗TMV分子标记辅助选择体系,本研究以高抗TMV烤烟品种Coker176和易感TMV烤烟品种Y3以及它们的BC1F1群体为试材,采用分离集团分组分析法(Bulked Segregation Analysis,BSA)和简单序列重复(Simple Sequence Repeat,SSR)标记技术,筛选与TMV抗性基因N紧密连锁的共显性SSR标记.通过对18764对SSR引物的分析表明,有396对SSR引物在两亲本间有多态,选出1对SSR引物TM508-007扩增出的标记与TMV抗性的N基因紧密连锁,遗传连锁距离为0.41 cM,证实获得的与TMV抗性基因N紧密连锁的共显性SSR标记稳定可靠,可用于烟草抗TMV分子标记辅助选择.%Tobacco mosaic virus (TMV) was one of the major diseases in tobacco (Nicotiana tabaccum L.) production.Separate progenies (BC1F1 population) from a cross between a resistant parent Coker176 and susceptible parent Y3 were used to establish TMV resistance gene N molecular marker-assisted selection system by bulked segregation analysis (BSA) method and simple sequence repeat (SSR) technology techniques.Among 18,764 SSR primer pairs,396 SSR primers were polymorphic between parents,while only one SSR marker TM508-007 amplified products was closely linked to TMV-resistant gene N and the genetic distance between TM508-007 and gene N was 0.41 cm.The co-dominant SSR marker screened in this study enriched the number and types of molecular markers in terms of tobacco's resistance to TMV,and they are expected to be used as marker-assisted selection in tobacco's resistance to TMV disease.