采用广西、江西和广东三地从澳大利亚引种互叶白千层茶树所提炼出来的茶树油做研究对象,先用精密分馏分离为若干组分,然后用气质联用仪(GC/MS)定量分析,确定每一组分的主要化学成分.同时用大肠杆菌和金黄色葡萄球菌作供试菌,分别检测茶树油各段馏分对两种菌的抑菌活性,对比研究各馏分化学成分与抑菌效果之间的关系.结果发现:三种茶树油的化学成分存在巨大差异,特征成分4-松油醇的质量分数从0.78％ ～43.59％不等,而1,8-桉叶素的质量分数则从2.14％ ～ 72.49％.三种茶树油的化学成分虽然显著不同,但是对大肠杆菌和金黄色葡萄球菌都有较强的抑菌活性,而且三种茶树油的有效抑菌物质都是1,8-桉叶素.而主要成分4-松油醇、γ-松油烯、α-松油烯和伞花烯等物质的含量与抑菌作用没有显著关联.%Three melaleuca alternifolia tea tree oil from Guangxi, Jiangxi and Guangdong province, they are introduced from Australia, were separately into different components by fractional distillation , then make sure the main chemical composition by GC/MS. By means of antibiotic experiment with colibacillus and staphylococcus aureus, through contrast research the antimicrobial activity of the different fraction. Results indicated that the chemical components of the three kinds of tea tree oil were in great diversity. The characteristic composition terpinen-4--ol accounted from0. 78% -43. 59% , while the noncharacteris-tic composition 1, 8-cineole accounted from 2. 14% -72. 49%. The three kinds of tea tree oil were in great diversity, but they all have strong antimicrobial activity to the Staphylococcus aureus and the colibacillus, the active constituent of the three kinds of tea tree oil were 1, 8-cineole. While the main component content, such as terpinen-4-ol, γ-Terpinen, α-Terpinen, p - Cymene. et, have no significant correlation with antibiotic activity.
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