溶藻细菌L7的高密度培养及溶藻活性物质提纯鉴定

摘要

Base on an indigenous algicidal bacteria L7, technical and process parameters for high cell density culture and the research and development of biological algicide were investigated. In the stage of high cell density culture, we selected the appropriate cell culture medium of bacteria L7 (carbon and nitrogen source are glucose +NH4Cl, C/N ratio is 3:1, pH 7. 5) , bacterial load (3. 1×107 cfu/mL) , DO (30% ( ± 10% ) ) and agitation speed ( 160 ( ± 10) r/min) through the setting of single factor experiment and orthogonal experiment, the application of shake flask and automatic fermentor. The process of purify and identify algicidal components of L7 included dialysis, gel chromatography, HPLC, MS and et al. Finally two algicidal components were received, the molecular weights were 588. 2 and 365. 0 respectively , and the algicidal effect of the latter one weas better.%针对一株溶藻细菌L7,探索溶藻细菌高密度培养的工艺参数及溶藻活性物质提纯鉴定的关键技术,为生物杀藻剂的研制奠定理论基础.在溶藻细菌L7的高密度培养阶段,通过设置单因素实验及正交实验、应用摇瓶及自动发酵罐筛选出适宜培养溶藻细菌L7的培养基(碳源葡萄糖、氮源氯化铵、C/N质量比3:1、初始pH值7.5)、细菌接种量3.1×107cfu/mL、DO [30％(±10％)]及搅拌速率[(160±10)r/min];在溶藻细菌L7溶藻活性物质的提纯鉴定阶段,通过透析、凝胶柱层析、高效液相色谱、液质联用仪等物质提纯鉴定手段,获得2种溶藻活性物质,相对分子质量分别为588.2、365.0,相较而言,相对分子质量为365.0的物质的溶藻作用更强.