VOC 增溶菌的发酵培养及增溶作用研究

摘要

To improve the water-solubility and biodegradability of VOCs,VOCs solubilization microor-ganisms were domesticated to make its produce biosurfactant to make VOC solubilize.Benzene and tolu-ene were selected as representative composition,such as solubilization activity and emulsifying rate as in-dex,the fermentation conditions,production regularity and solubilization effect of microorganisms of VOCs solubilization etc were studied.The result showed that the solubilization microorganisms that pro-duce BST were successfully domesticated.The optimum fermentation conditions for the production of BST was that the best carbon source concentration was 1.0 g/L and the optimal initial pH value was in the range of 6.95 ~7.05 and the optimum temperature range was in the range of 26 ~32 ℃.The solubiliza-tion bacteria had an obvious regularity for production of BST.The BST continue to increased rapidly dur-ing the fermentation of 0 ~36 h,and arrived at a maximum yield at 36 h.The fermentation liquid had good effect on the solubilization of benzene and xylene.The fermentation liquid had good effect on the solubilization of benzene and xylene.They were 2.7 times and 30 times of the pure medium,respective-ly.The emulsion droplets formed by benzene and BST were as an oil in water and it is soluble in water. This was more favorable for the biodegradation of VOCs.The bacteria producing BST were mostly gram negative bacilli.The BST was initially identified as a polysaccharide.The content of BST in the fermenta-tion broth was about 60 g/L.%选择苯和甲苯作为 VOCs 代表成分，以增溶活性和乳化率作为指标，研究增溶微生物的发酵条件、BST 产生规律及增溶效果等。结果表明，成功驯化产 BST 的增溶微生物。其最适发酵条件是：碳源质量浓度1.0 g／L，初始 pH 值6.95～7.05，温度范围26～32℃。增溶菌具有明显的产 BST 规律：在0～36 h 内持续快速增加，36 h 时，产生量最大。发酵液对苯和甲苯具有良好的增溶效果，分别是纯培养基的2.7倍和30倍。苯与BST 所形成的乳滴为水包油，易溶于水，有利于 VOCs 的生物降解。产 BST 的细菌大都是 G －短杆菌。