抗逆转录因子在百脉根中的功能分析

摘要

Two drought-resistant genes (PeDREB2a and HhERF2) were identified through functional analysis of the four transcriptional factor genes cloned from Populus euphraticaand Halimodendron halodendron,respectively.PeDREB2a and HhERF2 were two factors which had positive effects in plants against drought stress.Based on this,a series of plant expression vectors either harboring PeDREB2a or HhERF2 were constructed with a non-resistant marker gene and transferred into the Lotus genome using an Agrobacterium mediated method.Transgenic Lotus had strong tolerance to drought.This showed that transcription factors significantly improved drought resistance of L.corniculatus,and the survival rate of transgenic L.corniculatus was 100％ in a drought recovery water test.%为提高豆科牧草百脉根抗旱方面的能力,从沙生植物胡杨和铃铛刺中克隆了4个抗逆转录因子基因并进行功能分析,从中筛选了2个具有抗旱特性的基因PeDREB2a和HhERF2,构建了以磷酸甘露糖异构酶为筛选标记的单、双价植物表达载体,转化百脉根Leo品种,结果表明,转录因子基因明显改善了百脉根的抗旱特性,经过抗旱复水试验证明转基因百脉根成活率达到100％.