建立测定人血浆中倍他米松的LC-MS/MS方法.采用Venusil XBP C8(200 mm ×3.9 mm ID,5 μm)色谱柱,流动相为甲醇-水(含甲酸铵5 mmol·L-1)(80:20),流速0.4 mL·min-1;质谱仪离子源为电喷雾离子源(ESI),正离子模式检测,监测离子为393.3→355.2(倍他米松)和361.3→343.2(泼尼松龙,内标).血浆样本用乙酸乙酯处理.倍他米松在0.5～80.0 ng·mL-1线性关系良好(r=0.999 2),血浆低、中、高3种浓度(1.0,10.0,60.0ng·mL-1)平均提取回收率为88.24%,定量限为0.5 ng·mL-1.本方法操作简便、准确、灵敏,适用于复方倍他米松注射液人体药代动力学研究.%A sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of betamethasone in human plasma. The analyte was isocratically eluted on a Venusil XBP C8 column (200 mm ×3.9 mm ID, 5 μm) with methanol-water with a triple quad LC-MS/MS using ESI with positive ionization. Ions monitored in the multiple reaction monitoring (MRM) mode were m/z 393.3→355.2 for betamethasone and m/z 361.3→343.2 for prednisolone (IS). Betamethasone was extracted from 0.5 mL human plasma with ethyl acetate. The validation study demonstrated excellent precision and accuracy across the calibration range of 0.5 - 80.0 injection in healthy Chinese volunteers.
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