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缘管浒苔和羽藻氮、磷营养生理学研究

     

摘要

The studies on the uptake and growth kinetics of NO3- ,NH4+ 和 PO34- N in the clone of Enteromorpha linza and Bryopsis plumosa were conducted. The results suggested that NO3- and PO43- were taken up in the form of active uptake by the two alga, with NH4+ taken up by passive diffusion. The parameters of Vmax and straight slope for E linza were higher than that for B. plumose, which suggested that E. linza could be more favor to high N than B. plumosa. Moreover, the value of a (Vmax/Ks) for NO3- and NH4+ in E. linza was obviously higher than that in B. plumosa, which showed that E. linza could have more approachability to low N than B. plumosa. As for the uptaken of PO34- , E. linza may have higher P uptake capacities at high P and lower approachability to low P than B. plumosa due to the higher Vmax and lower a than B. plumosa. NO3 is the most favorite N form to meet the growth for both species, while NH4+ is more object to increase the accumulation of chlorophyll than NO3 . In addition, B. plumosa exhibited higher growth rate than E. linza under same N condition.%以无性繁殖系为材料,开展了缘管浒苔和羽藻对NO-3,NH+4和PO3-4的吸收动力学、生长动力学研究.吸收动力学研究结果表明缘管浒苔和羽藻对NO-3和PO3-4的吸收方式为主动运输,对NH+4的吸收方式为被动扩散.缘管浒苔对NO-3的最大吸收速率(Vmax)、对NH+4的吸收斜率都大于羽藻,说明缘管浒苔对高浓度的NO-3和NH+4具有更强的吸收能力.缘管浒苔吸收NO-3和NH+4的α值远大于羽藻,说明在低营养盐浓度时,缘管浒苔对NO3和NH+4的亲和力更强.在PO3-4的吸收中,羽藻的最大吸收速率(Vmax)远大于缘管浒苔,说明羽藻对高浓度PO3-4的吸收能力更强,但缘管浒苔的α值远大于羽藻,说明前者在低营养盐浓度时PO3-4的亲和力更强.生长动力学研究结果表明,硝酸氮是促进两种海藻快速生长的最适宜氮源形式,氨氮更易促进藻体叶绿素的积累.在相同氮营养条件下,羽藻表现出比缘管浒苔更强的生长优势.

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