NP-5 Alleviating Effects of Osthol on Scopolamine-Induced Cholinergic Impairments in Mice

摘要

Objective:To investigate the effect of osthol on the central cholinergic nerve circuit in AD model mouses,,which were established by intraperitoneal injection of scopolamine.Methods:60 health female rats were randomly divided into six groups.The sham operation group and model group were given intragastric administration of normal saline;the positive control group was given Aricept(3 mg·kg-1)intragastric gavage;The high,middle and low doses of the osthole groups were treated by intragastric administration of 50 mg·kg-1,25 mg·kg-1,and 12.5 mg·kg-1 osthole,respectively.30 minutes before the water maze test,except for the sham operation group who was given intraperitoneal injection of normal saline,other groups were intraperitoneally injected with scopolamine hydrobromide(3 mg·kg-1)until the experiment was completed.The ability of spatial learening and memory in mice was evaluted by behavioral experiments and changes and changes in the central cholinergic function of mice were examined The ability of spatial learning and memory in mice was evaluated by behavioral experiments and changes of function of the central cholinergic cricuis of mice were detected by molecular biology and pathology.Results:1.Effects on cholinergic nerve pathways:Osthole can reduce escape latency and search distance in dementia mice,while the osthol can increase the level of ACH in the central cholinergic circuits of dementia mice.2 effects on oxidative stress pathway:The activity of SOD in the model was obvious lower than that in the normal group,while the SOD activity of each dose of osthole was higher than that in of the model group.The content of MDA in the model group was obvious higher than that in the normal group,while each dose of osthole was lower than that of the model group.The activity of GSH-Px in the model group was significantly lower than that in the normal group,while the activity of GSH-Px in the osthol group was higher than that in the model group.Conclusion:The scopolamine-induced mouse model of denentia can cause cognitivedysfunction of mice and reduce the content of acetylcholine and acetylcholinesterase(AchE)in the central cholinergic circuit in mice of denentia,Osthole has effect on improving cognitivein dysfunction,increasing content of ACH and improving the activity of acetylcholinesterase(AchE)in the central cholinergic nerve circuit in dementia mice.