酶活性测定结果不确定度评估的一般原则

摘要

在实验的基础上总结了一套酶活性测定结果不确定度评估的一般方法和基本原则,包括实验条件的优化和建立原则、不确定度来源分析、鱼骨图的绘制、灵敏系数和不确定度分量的计算、标准合成不确定度及扩展不确定度的计算等.为国内各个领域酶活性测定结果的准确性与有效性和研制酶活性标准物质奠定了基础.%The general principle for uncertainty evaluation of enzyme activity measurement is presented based on previous experiment Totally five steps toward the uncertainty evaluation of enzyme activity measurement are introduced, including the principle of experimental condition selection and optimization, uncertainty source analysis, uncertainty component and combination graph drawing, sensitivity factor and uncertainty component calculation, standard combination uncertainty and expanded uncertainty calculation. The principle presented will lay a foundation for the accuracy and validity in the enzyme activity measurement and enzyme activity certified reference material production.