拟南芥6个酪蛋白激酶基因的组织表达及其生物信息学分析

摘要

The quantitative real-time RT-PCR was used to determine the tissue expression patterns of six casein kinase (CK) genes. The results showed that the above six genes were expressed ubiquitously in various organs and tissues, but the expression levels were different. The expressions of AT4GU340, AT3G23340, AT1C03930 and 473G03940 were highest in the flowers, and relatively lower in roots, stems, leaves and leafstalk. The expressions of ATX G04440 and AT4G26100 were highest in roots, and relatively lower in flowers, stems, leaves and leafstalk. Differentiation of base composition, nucleotide and amino acid among six CK genes were analysed. The results showed that the frequencies of six CK genes were A > T > G > C. The nucleotide homologies of the six CK genes were 60% to 83% , 20% to 79% for amino acid homologies. Analysis of putative promoter region of six CK genes identifies several regulatory elements related to stress response. The six CK genes might therefore be much related to plant stress tolerance.%利用实时荧光定量PCR技术研究6个酪蛋白激酶基因在不同组织中的表达特性.结果表明:6个基因在各个器官中均有表达,但表达量不同.AT4G14340、AT3G23340、AT1G03930和AT3G03940基因在花中表达量最高,在根中其次,在茎、叶和叶柄中的表达量最低；A T1G04440和AT4G26100基因在根中的表达最高,在花、茎、叶和叶柄中的表达量较低.对6个基因的碱基组成、核苷酸和氨基酸序列差异进行了比较.结果显示:它们的核苷酸组成情况为A＞T＞G＞C,核苷酸同源性在60％～ 83％之间,氨基酸同源性在20％～79％之间.对启动子区域进行分析,发现含有多种与逆境诱导相关的调控元件,推测这6个基因与植物耐逆性有一定关系.