Cadmium ( Cd ), a non-essential element, exhibits high levels of toxicity. Cd2+ exposure could inhibit plant growth and even result in cell death. Yet the mechanisms of Cadmium-induced programmed cell death ( PCD ) remain largely unknown. Using fluorescent probes molecular imaging and laser confocal scanning microscopy, the temporal and spatial characteristics of nitric oxide ( NO ) production during Cd2+ -induced PCD in Arabidopsis protoplasts were investigated in vivo and in real-time. Results demonstrated that high doses of Cd2+ strongly decreased cell viability and induced large amount of NO. After 12 h treatment with 100 μmol/L Cd2+ the NO content reached a maximum. NO was first generated in the mitochondrial regions of protoplasts and subsequently in chloroplasts and even whole cell as the treating time went on.To study the effects of laser on animal corneal proteins structure and explore the molecular mechanism of laser induced cornea injury, the rabbits were divided randomly into injury group and normal group, and fourier transform infrared spectroscopy ( FT-IR ) was applied to observe the change of proteins structure in the corneal epithelium and stro-ma. It reveals that there are significant spectral differences of the peak position and the percentages of absorption value for secondary protein structure after laser exposure. These results suggest that the secondary structure of corneal epithelium and stroma proteins have changed since the laser irradiation, which may lead to the decrease in proteins structural stability and the loss of proteins function.%镉是一种高度有毒的重金属,能够抑制植物生长,甚至导致死亡,但是其诱导细胞程序性死亡(PCD)的分子机制仍然不是很清楚.本文利用荧光探针分子成像和激光共聚焦扫描显微技术,以拟南芥叶肉细胞原生质体为材料,观察100 μmol/L CdCl2诱导PCD过程中一氧化氮(NO)的产生和亚细胞定位.结果表明高浓度CdCl2能够明显降低细胞活力,100 μmol/L CdCl2能够诱导大量NO产生,在处理12 h后NO产生达到峰值.亚细胞定位观察发现NO荧光首先和线粒体有共定位.随着处理时间的延长在叶绿体和胞质也观察到NO荧光.
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