毕赤酵母发酵产S-腺苷蛋氨酸的甘油-甲醇混合流加策略

摘要

在毕赤酵母发酵生产S-腺苷蛋氨酸(SAM)的诱导阶段,以不同甘油-甲醇比例的甘油-甲醇混合培养基进行诱导培养,结果表明以10% (w/v)甘油含量的甘油-甲醇混合培养基进行诱导培养时最有利于SAM的表达,SAM产量达6.09 g/L,比0%甘油含量条件下的SAM产量提高了20.4%.对诱导方式进行优化,先以100%甲醇诱导24 h,然后再连续流加10% (w/v)甘油含量的甘油-甲醇混合培养基,SAM产量可达7.94 g/L,在此基础上,进一步改进诱导方式,SAM产量得到进一步的提高,达到9.80 g/L.%Mixed carbon sources culture medium with different ratio of glycerol to methanol was evaluated in the S-ade-nosyl-L-methionine ( SAM ) fermentative production by recombinant Pichia pastoris. When the supplement mixed medium contained 10% ( w/v ) glycerol, SAM production reached 6. 09 g/L, which was 20. 4% higher than the production of feeding 0% glycerol contained medium. On that basis, two sets of glycerol-methanol mixed-feeding strategy were performed at the 7 L fermentor to improve SAM production. When 100% methanol was fed into bioreactor for 24 h before 10% ( w/v ) glycerol content of mixed medium was put into use, SAM production was raised to 7. 94 g/L. When different glycerol content of mixed medium was fed into bioreactor at five different sections during the methanol induction phase, SAM production reached the highest 9. 80 g/L.