为研究TiO2和CdTe量子点间荧光共振能量转移效率对PDT体外灭活HL60细胞的影响,本文以发射波长为407.8 nm的TiO2为供体,CdTe为受体,通过TiO2与CdTe超声混合构建荧光共振能量转移体系,研究了TiO2和CdTe量子点间荧光共振能量转移;其中体系中TiO2浓度为200μg/mL时,通过逐渐增加体系中CdTe浓度来观察供体TiO2荧光强度变化,根据Forster能量共振转移理论计算体系能量转移效率.之后将体系用于PDT体外灭活HL60细胞的实验研究,采用CCK-8法,结合酶联免疫检测仪进行细胞活性检测,得出不同浓度下体系的PDT灭活效率.发现当TiO2-CdTe体系荧光共振能量转移效率20.21%时,PDT灭活效率为53.75%,而当体系能量转移效率为6.77%时,灭活效率达到了71.54%,实验表明在一定浓度范围内,TiO2-CdTe混合体系荧光共振能量转移效率低时,PDT灭活效率更高.这可能是由于TiO2-CdTe之间能量共振转移低时,容易致使TiO2表面光生电子和空穴复合率降低,提高了二氧化钛的光催化活性,导致灭活效率增高.%A fluorescence resonance energy transfer system based on TiO2 and CdTe ultrasonic mix was built to study how fluorescence resonance energy transfer between TiO2 and CdTe quantum dots influence HL60 viability in PDT exper-iment.TiO2 with 407.8 nm emission wavelength was used as donor,and CdTe as acceptor.The intensity change of do-nor TiO2 was observed when the concentration of CdTe was increased in proportion,and the concentration of TiO2 re-mained at 200 μg/mL.The system energy transfer efficiency was calculated according to the theory of Forster energy transfer.The system was then applied in in vitro PDT experiment based on HL60 cells.With CCK-8 method,the cell vi-ability under different drug concentration was calculated.Our data showed that when the fluorescence resonance energy transfer efficiency of TiO2-CdTe hybrid system was 20.21%,the PDT inactivated efficiency was 53.75%; when the system energy transfer efficiency was 6.77%,the inactivated efficiency reached 71.54%.The results showed that low fluorescence resonance energy transfer efficiency promote higher PDT inactivation efficiency.This might be on account of the decrease of the recombination rate of electron-hole pair caused by the decline of fluorescence resonance energy trans-fer efficiency,which enhanced the photocatalytic activity of TiO2.
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