CONSTANS LIKE 7参与调控拟南芥生物钟

摘要

The circadian clock of plants can be trained by light/dark and cold /hot circles,and make it synchronized-with the environment.The circadian clock of plants is composed of input pathway,oscillator central and output pathway. The present study of circadian clock has revealed the basic composition of circadian clock,butthe operation mechanism and network of circadian clock need further research.CONSTANS LIKE 7(COL7)is one of the CONSTANS(CO)family genes.We used the real-time PCR,leaf movement to analyze the circadian clock of wild type (WT),col7 mutant and COL7 overexpression lines (COL7-OX-1 0 and COL7-OX-1 1 ).The results showed that COL7 is regulated by circadian clock and also involved in regulating circadian clock.%植物的生物钟节律可以被环境中的光／黑暗以及冷／热循环所诱导，并使其与环境同步。植物生物钟由输入途径、中央振荡器、输出途径组成。目前对植物生物钟的研究已经揭示生物钟最基本的组成，但是关于生物钟的运作机理及网络还需要进一步研究。CONSTANS LIKE 7（COL7）是 CONSTANS（CO）的家族基因。以拟南芥野生型（wild type，WT）、突变体 col7以及 COL7过量表达转基因株系 COL7-OX-10和 COL7-OX-11为材料，利用定量 PCR、叶片运动等方法，分析 COL7是否受生物钟调控以及 COL7是否参与调控生物。实验结果显示：COL7不仅受生物钟调控，同时也参与调控生物钟。