目的 评价脂多糖(LVS)诱发大鼠慢性阻塞性肺疾病(COPD)模型的可行性.方法 气管内滴入脂多糖或生理盐水,每周1次,共8周.测定大鼠的气道阻力(R<,L>)和肺动态顺应性(C<,dyn>),计数大鼠支气管肺泡灌洗液(BALF)中白细胞总数及分类,肺组织病理切片行HE和AB-PAS染色,并测定肺组织粘蛋白(MUC5AC)的含量.结果 模型组大鼠R<,L>明显升高(87,5%),C<,dyn>显著下降(16.4%);BALF中白细胞总数及分类中的中性粒细胞、淋巴细胞和单核巨噬细胞数均明显高于对照组;光镜下可见病变呈慢性支气管炎及肺气肿样改变.结论 反复气管内滴入LPS可用于制备大鼠COPD模型,其肺功能、BALF细胞学及病理学改变符合人类COPD表现,可以用于实验研究.%Objective To establish and evaluate the feasibility of a rat model of chronic obstructive pulmonary disease (COPD) induced by intratracheal instillation of lipopolysaccharide (LPS). Methods LPS or saline was instilled intratracheally in rats once a week for consecutive 8 weeks. The rats were anesthetized and taken for the measurement of lung resistance ( RL ) and dynamic compliance ( Cdyn ). Total and differential white blood cell counts in bronchoalveolar lavage fluid (BALF) were carried out. The pathological changes of the lung tissues were observed using HE and AB-PAS staining, and the mucin 5AC content was measured. Results Intratracheal instillation of LPS caused a significant increase in RL(87. 5％ ) and decrease in Cdyn( 16. 4％ ). Total and differential white blood cell counts in BALF such as neutrophils,lymphocytes and macrophages were higher in the LPS-treated rats. Chronic bronchitis and emphysematous changes were observed as well. Conclusion A typical rat model of COPD can be induced by repeated intratracheal instillation of LPS, showing many features of human COPD in the lung function, BALF cytologic and pathologic changes.
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