异育银鲫消化道微生物群落对恢复投喂的响应

摘要

In this study, we applied PCR-DGGE fingerprinting to investigate the effects of refeeding on the diversity of intestinal microflora in allogynogenetic crucian carp. Fishes were reared in a semi-recirculating system until maturation at Institute of Hydrobiology. After the 30-day starvation, fishes were divided into two groups (the hungry group and the feed group). Three fishes were blindly selected from the hungry group for the extraction of the microbial DNA with the traditional Phenol-chloroform method. In the meanwhile, fishes in the feed group resumed to be fed. Three fishes in the feed group were blindly selected for extraction of the microbial DNA after 25 days. The PCR-generated bacterial DNA products were separated using a polyacrylamide gel as it exhibited increasingly higher concentrations of chemical denaturant. Correspondence Analysis (CA) and clustering based on Jaccard index was used to analyze the similarity of the samples. The results showed that the feed group displayed less bands than the hungry group. There was significant diffe-rence in the deleted bands between the feed group (B1, B2, B5, B6, B7 and B23) and the hungry group (B11, B14, B16, B17 and B21), so we reasoned that the similarity of microflora between the two groups was low. The variance analysis showed that the variance within the feed group was larger than that within the hungry group. In conclusion, our results suggested that refeeding had strong effects on the diversity of intestinal microflora in allogynogenetic crucian carp.%以实验室养殖的异育银鲫(Carassius auratus gibelio)“中科3号”成鱼为对象，利用PCR-DGGE技术比较研究了其经饥饿与恢复投喂后消化道微生物多样性的变化。实验共检测到23条谱带，其中饥饿组、投喂组谱带数分别为18条和17条(共有谱带12条)，投喂组大部分谱带亮度高于饥饿组。基于PCR-DGGE所得到的谱带进行的 CA (Correspondence Analysis)分析和聚类都表明饥饿组与投喂组消化道微生物存在明显不同。结果表明，在充分饥饿处理后，恢复饲料投喂可导致消化道微生物种类减少及微生物平均密度的增加，同时对消化道微生态系统内微生物种类组成有显著影响。研究为进一步阐述消化道微生物功能基因奠定了基础，也为水产鱼类肠道菌群研究提供一些基础数据。