甘薯蚁象对不同甘薯品种植物挥发物的EAG和嗅觉反应

摘要

[目的]为筛选对甘薯蚁象Cylasormicarius (Fabricius)有引诱作用的植物挥发性物质.[方法]采用顶空气体采样法收集5个甘薯品种的植物挥发物,通过气相-质谱联用系统(GC-MS)进行物质鉴定及定量分析,测定甘薯蚁象成虫对候选挥发物的触角电位(EAG)反应和嗅觉趋向选择行为.利用Y型嗅觉仪测定甘薯蚁象成虫对5个甘薯品种茎叶的嗅觉反应.[结果]5种甘薯品种的植物挥发物种类和含量有所差异.7种候选挥发物(罗勒烯、柠檬烯、壬醛、β-石竹烯、律草烯、法尼烯和乙酸-顺-3-己烯酯)均引起甘薯蚁象雌、雄成虫的EAG反应.其中,雌虫对柠檬烯、壬醛和β-石竹烯的反应相对值较大;而引起雄虫反应相对值较大的挥发物为罗勒烯、柠檬烯、葎草烯.在趋向行为反应中,柠檬烯和壬醛对甘薯蚁象雄虫的吸引作用高于对照,而雌虫对葎草烯、柠檬烯有极显著的趋向选择.甘薯蚁象成虫对5种茎叶的反应率为40.90％ ～65.21％,且5个甘薯品种的茎叶挥发物均没有显著的引诱作用(P＞0.05).[结论]甘薯蚁象雌、雄成虫对甘薯植物挥发物具有不同的EAG及趋向行为反应,气味化合物柠檬烯与甘薯蚁象偏爱选择密切相关.%[Aim] This study aims to screen plant volatiles attracting Cylas formicarius.[Methods] Plant volatiles of five sweetpotato cultivars were collected by headspace adsorption device and then identified and quantified on GC-MS.EAG response and taxis of C.formicarius adults to candidate volatiles from the sweetpotato plants were assayed.And the olfactory responses of C.formicarius adults to volatiles emanating from stems and leaves of five sweetpotato cultivars were measured by using a Y-tube olfactometer.[Results] The GC-MS results showed that the types and contents of volatiles collected from the five sweetpotato cultivars were different.Seven candidate volatiles (ocimene,limonene,nonanal,β-caryophyllene,humulene,farnesene,and (Z)-3-hexen-1-olacetate) elicited EAG responses in both male and female adults of C.formicarius.The EAG responses of female adults of C.formicarius to limonene,nonanal and β-caryophyllene were significantly higher than to the other chemicals tested,while those of males to ocimene,limonene and humulene were significantly higher than to other chemicals tested.In taxis assay,limonene and nonanal attracted male adults,and humulene and limonene attracted females.The response rates of C.formicarius adults to volatiles from stems and leaves of the five sweetpotato cultivars were 40.90％ ～65.21％,and the volatiles emitted from sweetpotato stems and leaves of the five cultivars had no significant attractivity to all adults (P ＞ 0.05).[Conclusion] Male and female adults of C.formicarius show different EAG and olfactory behavioral responses to the volatiles from the sweetpotato,and the volatile limonene is closely related to the preference of C.formicarius.