海南烟粉虱田间种群隐种鉴定及对溴氰虫酰胺和氟吡呋喃酮的敏感性检测

摘要

[目的]明确海南烟粉虱Bemisia tabaci田间种群对2种新型杀虫剂溴氰虫酰胺和氟吡呋喃酮的敏感水平,为杀虫剂的合理施用与烟粉虱的田间抗性监测提供参考.[方法]以2017年1月采自海南省6个地点烟粉虱田间种群为材料,利用线粒体细胞色素氧化酶I基因(mtCOI)测序及mtCOI PCR-RFLP分子标记对采集种群隐种进行鉴定;采用浸叶法测定溴氰虫酰胺和氟吡呋喃酮2种药剂对各地区田间种群的致死中浓度(LC5o).[结果]在海南省6个烟粉虱田间种群中,三亚市吉阳区种群为MEAM1隐种,澄迈县永发镇种群和乐东黎族自治县冲坡镇种群以MED隐种为主的MEAMl(＜10％)与MED隐种(分别为94.6％和92.9％)混合种群,其他3个田间种群均为MED隐种.与溴氰虫酰胺敏感种群相比,除乐东黎族自治县十所种群已处于低水平抗性(RR:5～10倍),其他5个种群均处于敏感水平(RR ＜5倍);所有6个田间种群对氟吡呋喃酮均处于敏感水平(RR ＜3倍).[结论]溴氰虫酰胺和氟吡呋喃酮可作为烟粉虱防控的新药剂,并可作为其抗性治理的替代药剂,但同时应密切关注烟粉虱对溴氰虫酰胺抗性水平变化情况.%[Aim] Determining the susceptibility of field populations of the whitefly,Bemisia tabaci in Hainan,China to two novel insecticides,cyantraniliprole and flupyradifurone,can help to provide the reference for the rational application of the insecticides and resistance monitoring of its field populations.[Methods] The cryptic species identity of field populations of B.tabaci collected from six localities in Hainan Province,southern China in January,2017 was identified by sequencing of mitochondrial cytochrome oxidase I gene (mtCOI) and PCR-RFLP analysis of mtCOI.The LC50 values of cyantraniliprole and flupyradifurone against these populations were measured using leaf-dip method.[Results] Among the six populations,the population from Jiyang,Sanya city was identified as the cryptic species MEAM1,those from Yongfa,Chengmai county and Chongpo,Ledong county were identified as the mixed populations of the cryptic species MEAM1 (＜ 10％) and MED (94.6％ and 92.9％,respectively),and the other three were identified as the cryptic species MED.By comparison with the LCso value of cyantraniliprole against the susceptible population,the population from Shisuo,Ledong county,had developed low-level resistance to cytraniliprole (RR:5-10),while the other five were susceptible (RR ＜ 5).And by comparison with the LC50 values of flupyradifurone,all the six field populations were susceptible (RR ＜ 3).[Conclusion] Cyantraniliprole and flupyradifurone can be used as candidate insecticides in the control and resistance management of B.tabaci,and more attention should be paid to the resistance development of B.tabaci to cyantraniliprole in the field.