草菇漆酶基因在子实体形成过程中的表达分析

摘要

为了探明草菇(Volvariella volvacea)漆酶基因的功能，采用实时荧光定量PCR方法对11个漆酶编码基因(lac1-11)在子实体不同发育阶段的表达水平进行研究，结果表明：草菇漆酶在针头期、纽扣期、蛋形期、伸长期和成熟期均发生转录；表达趋势分为三类：lac1、lac5、lac7和lac8表达量针头期最高；lac3、lac6和 lac11从针头期至蛋形期逐渐升高，自伸长期表达量有明显下降；lac2、lac4、lac9和 lac10在纽扣期最高，随后表达量逐渐降低，其中lac9与lac10在成熟期又有了小幅度的提高。这些结果说明草菇不同的漆酶在子实体发育中可能起到不同的作用。%Quantitative real-time PCR was used to analyze the relative expression levels of 1 1 laccase genes (lac 1-11)at five different stages (pinhead,button,egg,elongation and mature)of Volvariella volvacea fruit body development.Expression levels of lac1,lac5,lac7 and lac8 gradually decreased from the pinhead stage whereas expression levels of lac3,lac6 and lac11 gradually increased from the pinhead stage to the egg stage before decreasing significantly at the elongation stage.Highest expression levels of lac2,lac4,lac9 and lac10 were recorded at the button stage.Expression levels then decreased gradually but,in the case of lac9 and lac10,increased again at the maturation stage.Our data indicated that the 11 laccase genes play different roles during the formation of the V.volvacea fruit body.