“沪农灵芝1号”聚酮合酶特异性分析

摘要

Genome-wide analysis of secondary metabolite (SM) clusters in Hunong Ganoderma lucidum 1 (G.lucidum SH) identified five backbone genes and three SM clusters of polyketide synthases (PKS).All three SM clusters were located in the scaffold terminals,and bioinformatics analysis revealed that two,PKS7 and PKS9,were present only in G.lucidum SH.Only the backbone gene of the PKS7 SM cluster (PKS7C) had complete functional motifs and single-copy orthologous features.Functional annotation genes involved in the biosynthetic pathway for unsaturated fatty acids were enriched in PKS7C.Our data show that the PKS7C SM cluster was specific for G.lucidum SH and possibly represents the molecular features of high stress resistance.%对“沪农灵芝1号”(简称沪灵芝)(Ganoderma lucidum SH)等10个代表性的担子菌物种进行全基因组次级代谢物(secondary metabolite,SM)簇分析,结果显示沪灵芝聚酮合酶(polyketide synthases,PKS)的骨架基因以及SM簇的数量分别为5和3,高于其它物种,并且都位于支架的末端.通过生物信息学分析发现,PKS7和9的SM簇只在沪灵芝中分布;只有PKS7的SM簇(PKS7簇)的骨架基因具有完整的Ⅰ型PKS功能模块;只有PKS7簇的骨架基因具有单拷贝直系同源性;PKS7簇的功能注释基因在不饱和脂肪酸生物合成通路中富集.这些结果表明PKS7簇为沪灵芝特异性的SM簇,可能代表了沪灵芝抗逆性强的分子特征.