COPD模型大鼠不同分期肺脏损伤程度与肝损害的相关性研究

摘要

目的:探讨COPD模型大鼠不同分期肺脏损伤程度与肝损害的相关性.方法:清洁级健康雄性Wistar大鼠24只,随机分成3组:正常组(A组),COPD模型组(B组)及COPD急性加重组(C组),每组8只.采用改良熏香烟加气管内注脂多糖的方法建立大鼠COPD模型,在COPD造模结束后第二天滴加脂多糖建立COPD急性加重模型.比色法检测肝功能指标,大鼠肝、肺组织匀浆中SOD活性和MDA含量,ELISA法检测肺组织中MMP-9、IL-8、TNF-α含量.结果:与A组比较,B组和C组肺组织SOD酶活性显著下降(P＜0.01),而MDA含量显著升高(P＜0.01),B组与C组比较有统计学意义(P＜0.05)；B组肝组织SOD酶活性下降不显著,而MDA含量显著升高(P＜0.01),C组SOD酶活性显著下降(P＜0.01),而MDA含量显著升高(P＜0.01),与B组比较有统计学意义(P＜0.05).肺组织MMP-9、IL-8、TNF-α含量显著升高(P＜0.01),C组比B组含量增高(P＜0.05).与A组比较,B组大鼠ALT升高(P＜0.05)、TBIL显著升高(P＜0.01),ALB下降(P＜0.05),AKP无统计学意义.C组大鼠ALT、TBIL、AKP显著升高(P＜0.01),ALB显著下降(P＜0.01)；与B组比较,C组ALT升高(P＜0.05)、TBIL显著升高(P＜0.01),ALB下降(P＜0.05),AKP升高(P＜0.05).结论:COPD状态下,肺脏损伤程度与肝损害呈相关性,即肺脏损伤程度越严重,肝损害越明显.%Objective: To observe the correlation between pulmonary injury degree and hepatic lesion at different stages of COPD. Methods; Divide the rats into three groups randomly:the blank group( A) ,the COPD group(B)and the acute exacerbation of COPD group(C) , eight rats in each group. Then establish the model of COPD by the improved passive smoking with LPS dropped into the trachea. Based on the model of COPD, drop LPS into the trachea again to establish the acute exacerbation model of the COPD. Then test the liver function, activity of SOD,content of MDA in the lung and liver homogenate with colorimetric method, detect the content of MMP - 9, IL - 8 and TNF - alpha in the lung homoge-nate by the ELISA assay. Results:Compared with group A, in group B and C ,the activity of SOD in the hung homogenate all descended significantly ( P <0.01) , and the content of MDA significantly increased (P <0. 01); compared with group B,group C was statistically significant(P <0. 05) ; in Group B, the activity of SOD in the liver homogenate declined slightly,with no statistical significance, and the content of MDA significantly increased(P <0. 01) ;In group C, the activity of SOD declined significantly (P <0.01) , and the content of MDA significantly increased(P <0. 01) , and compared with group B, group C was statistically significant( P <0.01). The content of MMP - 9, IL - 8 and TNF - alpha in hung all rose significantly (P <0. 01) , and group C was higher than group B(P<0.05). Compared with group A, in group B ,the content of ALT was higher(P <0. 05) , TBIL significantly increased(P <0. 01) ,while ALB dropped (P <0.05) , the AKP of group B had no statistical significance. In group C,the content of ALT,TBIL and AKP all rose significantly(P <0. 01) , ALB descended obviously. Compared with group B,the contents of ALT and AKP were higher (P <0. 05) , TBIL significantly increased( P < 0. 01) , ALB dropped( P < 0. 05 ) . Conclusion: At the state of COPD, hepatic lesion has a correlation with the degree of pulmonary injury. That is, the more serious the degree of damage to the lung is, the more obvious hepatic lesion it is.