乌芪通络胶囊对糖尿病周围神经病变大鼠背根神经节 JNK1／2和 p38 MAPK蛋白表达的影响

摘要

Objective:In order to explore the mechanism of Wuqi Tongluo capsule on diabetic peripheral neuropathy ( DPN) from oxidative stress aspect , we observed the protein expression of JNK 1/2 and p38MAPK in dorsal root ganglion (DRG) of DPN rats.Methods:80 male SD rats were randomly divided into two groups:model group(66 rats) and nor-mal group(14 rats) .The model group was molded with STZ Intraperitoneal injection , and obtained 51 successful mod-els.The DPN model group was randomly divided into model group , Wuqi Tongluo capsule group and Methycobal group , with 17 rats in each group .After eight weeks ’ administration , extract total protein from L4/L5 ganglion of rats ’ DRG. The JNK1/2 and p38 MAPK expression levels in DRG of each group were determined by western blot .Results:The pro-tein expression of JNK1/2 and p38MAPK in model group increased obviously than that in the normal group (P<0.05). Wuqi Tongluo capsule group and Methycobal group obviously decreased compared with the model group , and the differ-ence was statistically significant ( P<0 .05 ) .Conclusion:Wuqi Tongluo capsule can decrease the protein expression of JNK1/2 and p38MAPK in DPN rats, which may be one of mechanisms of Wuqi Tongluo capsule in the treatment of dia-betic peripheral neuropathy from oxidative stress aspect .%目的：研究乌芪通络胶囊对糖尿病周围神经病变大鼠背根神经节中JNK1/2和p38 MAPK蛋白表达的影响，从氧化应激的角度探讨乌芪通络胶囊治疗糖尿病周围神经病变的机制。方法：实验用雄性SD大鼠80只，随机选择14只为正常对照组，其余66只用来造模。后者经腹腔注射链脲佐菌素（ STZ），成功造模51只。将造模成功的大鼠随机分为模型组、弥可保组及乌芪通络胶囊组，每组17只。弥可保组和乌芪通络胶囊组连续给药8周，对照组和模型组正常饮食8周，将8周后的四组大鼠处死，取背根神经的L4/L5神经节，提取总蛋白，采用Western杂交的方法检测背根神经节中JNK1/2和p38 MAPK蛋白的表达水平。结果：模型组JNK1/2和p38 MAPK蛋白的表达比正常组显著增高（ P＜0．05）。乌芪通络胶囊组和弥可保组JNK1/2和p38MAPK蛋白表达较模型组显著降低，差异有统计学意义（P＜0．05）。结论：乌芪通络胶囊可降低大鼠背根神经节中的JNK1/2和p38 MAPK蛋白的表达，这可能是乌芪通络胶囊从氧化应激方面治疗和缓解糖尿病周围神经病变各种症状的机制之一。