The ROS metabolize and root growth of Vigna unguiculata L. cultivar 'T6' (Al-tolerant) and 'S3'(Al-sensitive) root tip was measured to analyze the relative between Al tolerance and ROS metabolize. The results show that: (1) The root length of ' T6 ' and ' S3 ' was inhibited with the increase of Al, the decrease of 'S3' was larger than that of 'T6'. (2)The superoxide anion radical (O2- ),H2O2 content,MDA content and plasma-membrane permeability of ' T6' and 'S3' root tip increase significantly by Al increase from 0 to 500 μmol · L1,but 'S3' made more increase than that of 'T6' (3)The SOD,POD,CAT,APX, GR, AsA,GSH of 'T6' and 'S3' root tip increased after exposure to 0 to 500 μmol · L-1 Al. The increase range of SOD of 'S3' root tip was higher than that of 'T6' ,but increase range of POD,CAT,APX of 'S3' root tip were lower than that of 'T6'. We presume that 'S3' root tip accumulated more ROS and increased the oxidative stress,then result in the inhibition of root growth.%选用豇豆铝敏感品种‘S3’和耐铝品种‘T6’为试验材料,研究不同耐铝性豇豆根系伸长和根尖活性氧代谢变化的差异,探讨铝胁迫下不同耐铝性豇豆在活性氧代谢上的差异及其与豇豆耐铝性的关系.结果表明:(1)随着铝处理浓度的增加,2个品种根系伸长均受到抑制,且‘S3’受到的抑制程度大于‘T6’.(2)随着处理铝浓度的升高,2个豇豆品种的根尖O2-·产生速率、H2O2含量、MDA含量及质膜透性都显著增加,且‘S3,的增加幅度大于‘T6’.(3)铝胁迫处理下,2个豇豆品种幼苗根尖超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)活性都有所上升,其中铝敏感品种‘S3’的SOD活性上升幅度比耐铝品种‘T6’高,而POD、CAT、APX的上升幅度比耐铝品种‘T6’低.研究认为,铝胁迫下铝敏感品种‘S3,内活性氧物质过量积累而导致氧化胁迫,使细胞的脂质过氧化程度加剧,最终严重影响根系的生长.
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