铝胁迫
铝胁迫的相关文献在1992年到2022年内共计374篇,主要集中在农作物、植物学、园艺
等领域,其中期刊论文356篇、会议论文7篇、专利文献109740篇;相关期刊142种,包括西北植物学报、农业环境科学学报、广西农业生物科学等;
相关会议7种,包括中国草学会草坪专业委员会第十五次学术研讨会 、第三届(2014)中国草业大会、中国草学会青年工作委员会2014年大会暨青年科学家论坛等;铝胁迫的相关文献由1018位作者贡献,包括刘鹏、徐根娣、刘强等。
铝胁迫—发文量
专利文献>
论文:109740篇
占比:99.67%
总计:110103篇
铝胁迫
-研究学者
- 刘鹏
- 徐根娣
- 刘强
- 何龙飞
- 李昆志
- 陈丽梅
- 何虎翼
- 贺根和
- 吴玉环
- 安渊
- 李晓红
- 李蕴
- 林思祖
- 柳正葳
- 肖宜安
- 蔡妙珍
- 钱莲文
- 刘大林
- 玉永雄
- 王爱勤
- 肖祥希
- 胡雪华
- 郑阳霞
- 黎晓峰
- 刘友良
- 周鹏
- 孙境蔚
- 张永福
- 李创珍
- 沈仁芳
- 沈振国
- 贺立源
- 金婷婷
- 陈海霞
- 马丽
- 龙婉婉
- 刘星辉
- 吴亚
- 吴文杰
- 张卫红
- 张美艳
- 张英俊
- 曹光球
- 杨宗武
- 沈宏
- 王凌晖
- 王琳
- 胡萃
- 薛世明
- 郦枫
-
-
苏启陶;
钱豪;
胡伟涛;
游媒;
罗轶;
周林霞
-
-
摘要:
为探究加拿大一枝黄花(Solidago canadensis)在不同浓度下对盐胁迫和铝胁迫的耐受性,以加拿大一枝黄花为材料,采用室内生物测定的方法,利用0、200、400、800、1600、3200 mg/L不同浓度NaCl和AlCl_(3)溶液处理加拿大一枝黄花种子,并对其萌发特性进行比较研究。结果表明:(1)加拿大一枝黄花种子的发芽率随着NaCl浓度的增加而降低,与对照相比,800 mg/L浓度的NaCl溶液对其发芽率和发芽势产生显著抑制作用(P<0.05),当浓度为1600~3200mg/L时,发芽率和发芽势极显著降低(P<0.01),当浓度为800~3200 mg/L时,加拿大一枝黄花发芽指数极显著降低(P<0.01);(2)加拿大一枝黄花种子发芽率随着AlCl3浓度的增加而降低,与对照相比,200~3200mg/L的AlCl_(3)溶液对其发芽率、发芽势及发芽指数受到极显著抑制作用(P<0.01)。(3)加拿大一枝黄花种子对盐胁迫具有相对更强的耐受性。
-
-
郑开敏;
肖家昶;
马俊英;
贺茂林;
格桑;
郑阳霞
-
-
摘要:
为研究外源柠檬酸对豆瓣菜铝胁迫的缓解作用,以湖北豆瓣菜和北京豆瓣菜为试验材料研究了不同质量浓度(0 mg/L、2 mg/L、10 mg/L、25 mg/L、50 mg/L和150 mg/L)柠檬酸对1 mmol/L铝胁迫下豆瓣菜生长及生理特性的影响。结果表明,铝胁迫抑制了湖北豆瓣菜和北京豆瓣菜的生长,减小了植株株高(分别减小44.44%、39.09%)、茎粗(34.49%、5.63%)和根长(44.61%、40.66%),加剧了叶片膜脂过氧化作用,渗透调节物质大量积累,抗氧化酶活性提高,促进了植株对铝的吸收,抑制了叶片对N、P、K、Ca、Mg、Fe、Zn、Cu的积累。从生长指标来看,铝对湖北豆瓣菜的毒害作用大于北京豆瓣菜。外源添加柠檬酸后,湖北豆瓣菜和北京豆瓣菜均表现为生长状况有所改善,丙二醛含量降低,抗氧化酶活性维持较高水平,铝含量下降,并且施用柠檬酸促进了豆瓣菜对各营养元素的吸收。湖北豆瓣菜在2~25 mg/L柠檬酸质量浓度下解毒效果最佳,在150 mg/L柠檬酸质量浓度下缓解效果受到抑制甚至消失。北京豆瓣菜则在10~50 mg/L柠檬酸质量浓度下解毒效果最佳。所以,适宜质量浓度的外源柠檬酸能有效缓解铝胁迫下豆瓣菜的生理损伤。
-
-
段宏利;
蒋凌雁;
陈志坚;
黄春琼
-
-
摘要:
为了探究狗牙根(Cynodon dactylon(L.)Pers.)耐铝种质及敏铝种质根系生长和营养元素对铝胁迫的响应。本研究以狗牙根耐铝种质A22和敏铝种质A326作为试验材料,在500μM AlCl_(3)(pH值4.0)溶液中设计了3个处理时间(0 h,12 h,24 h),在24 h处理时间点设计了4个铝处理浓度,分别为0(CK),250,500,750μM AlCl_(3)(pH值4.0)。通过对相对根长、根系苏木精染色、根系铝含量和10种营养元素的测定,结果表明,在不同铝胁迫下,A22根系伸长受抑制的程度均小于A326;A326根系苏木精染色深于A22,且根系铝含量高于A22;营养元素P,Ca,Mg,K,Fe,Mn,Cu,Zn和B的含量在A22和A326中都受到了不同程度的抑制,且与两个种质中根系铝含量呈负相关。初步了解铝胁迫下狗牙根在营养生理上的耐铝机制,有利于在酸性土壤中更好地利用狗牙根,为培育耐酸铝的草品种提供初步的理论基础。
-
-
徐仕琴;
陈姣;
张永福;
莫丽玲
-
-
摘要:
为探讨5-氮胞苷对铝胁迫下葡萄植株生长及耐铝相关生理机制的响应,以‘水晶’葡萄扦插苗为试材,在20 mmol·L^(-1)硫酸铝胁迫下喷施不同浓度的5-氮胞苷,35 d后测定植株的形态指标及生理指标。结果表明,铝胁迫下植株茎叶萎蔫,株高和根系生物量下降,MDA剧增,叶绿素含量下降,SOD和POD的活性均被减弱,铝大幅度积累,抑制氮、磷、钾的转运。铝胁迫下喷洒5-氮胞苷可使株高、根鲜质量、根长、根体积提高,MDA含量降低的同时使得叶绿素含量增加,SOD和POD活性显著增强,铝的积累缓解,增强了氮、磷、钾的转运。其中,铝胁迫下喷洒100μmol·L^(-1)的5-氮胞苷的处理效果最显著,可减缓铝胁迫对‘水晶’葡萄植株的毒害从而提高其耐铝性,促进植株和根系生长,增强营养物质的积累以及膜脂过氧化程度。
-
-
叶艳英;
张冰冰;
汤泳萍;
周劲松;
罗绍春;
尹玉玲
-
-
摘要:
通过室内培养皿试验,探究了7个不同浓度铝离子胁迫对3个芦笋品种种子萌发的影响。结果表明:铝离子浓度对3个芦笋品种种子的相对活力指数、胚根长、胚芽长、鲜重影响显著,表现为极显著的负相关关系,而对胚根毒害指数和胚芽毒害指数表现为极显著的正相关关系。通过建立回归方程,以胚根毒害指数下降到50%时的铝离子浓度为种子耐铝的临界浓度,求得井冈红、井冈111和京绿芦1号的耐铝临界浓度分别为512.70、604.07、507.55 mg/L。
-
-
赵恩华;
谢建平;
张钰靖;
安渊;
周鹏
-
-
摘要:
以紫花苜蓿品种WL-525HQ为材料,用发根农杆菌介导毛状根转化,分析毛状根分化率及转化率,筛选最优的转化方案,以快速获得紫花苜蓿转基因材料。结果表明:当发根农杆菌侵染苜蓿叶片的菌液浓度OD_(600)达0.5时,毛状根分化率可达72%;筛选培养基中添加5~8mg/L潮霉素时,毛状根的分化率及转化率都相对较高,分别为30.7%~33.2%和59.7%~67.9%,转化方案可靠;在以毛状根为外植体诱导全株时,采用羧苄青霉素能够有效抑制细菌,5mg/L潮霉素对愈伤组织的分化效果最好。优化了以毛状根为外植体探究紫花苜蓿全株的转化方案,并应用于耐铝基因MsLEA2研究,为紫花苜蓿功能基因研究提供了一种快速有效的手段。
-
-
杨文敏;
严浙楠;
毛纪隆;
倪晓菁;
黄家妮;
潘蕾蕾;
张雅琦;
娄赛炜;
杨颜裴;
张丽宇;
刘鹏
-
-
摘要:
为探究铝胁迫对菊芋根系分泌物的影响以及外源水杨酸(SA)的缓解作用,该文以耐铝型南京菊芋和铝敏感型资阳菊芋为试验材料,采用土培法,设置铝浓度500μmol·L^(-1),分析了不同浓度(10、100、1000μmol·L^(-1))SA对铝胁迫下菊芋根系分泌物中有机酸、氨基酸以及根尖相关代谢酶活的影响。结果表明:(1)单铝胁迫会导致菊芋根系分泌物中柠檬酸、草酸、苹果酸浓度升高,且南京菊芋升高幅度大于资阳菊芋;柠檬酸合酶和苹果酸脱氢酶在单铝胁迫下活性增强;脯氨酸含量显著提升,总氨基酸浓度均显著减少。(2)外源SA加入后,南京菊芋根系分泌的柠檬酸、草酸、苹果酸浓度均得到不同程度提高,但经高浓度(1000μmol·L^(-1))SA处理后资阳菊芋根系分泌草酸显著降低,且在各浓度SA处理下苹果酸浓度均无明显变化;柠檬酸合酶活性出现不同程度的增强,但对南京菊芋根尖中苹果酸脱氢酶活性影响不大,且高浓度(1000μmol·L^(-1))SA处理后显著降低了资阳菊芋根尖中苹果酸脱氢酶活性;脯氨酸含量显著下降,从总氨基酸浓度变化来看,南京菊芋在高浓度(1000μmol·L^(-1))SA、资阳菊芋在低浓度(10μmol·L^(-1))SA处理下得到最大缓解效果。因此,菊芋通过分泌有机酸应对铝毒侵害,外源SA可促进菊芋根系有机酸代谢速率,分泌更多的有机酸来缓解铝胁迫,这种缓解效果在耐铝性相对较强的南京菊芋中表现更好。
-
-
沈雪峰;
卢文涛;
陈勇
-
-
摘要:
为有效降低铝毒害,探讨铝胁迫下花生幼苗根系代谢变化,以花育23(H,铝敏感型)和粤油7号(Y,耐铝型)两个花生品种为材料,基于超高效液相色谱-串联质谱法(UPLC-MS/MS)的广泛靶向代谢组学,研究铝胁迫对花生根系的影响。结果发现,从两个花生品种的幼苗根系中检测出416种代谢产物,与对照(HC)相比,花育23(HA)共筛选出155个差异代谢物(即HC vs HA),其中上调代谢物为27种,下调为128种;粤油7号(即YC vs YA)共筛选出109个差异代谢物,其中上调代谢物为28种,下调为81种。铝胁迫下两个花生品种幼苗根系之间(即HA与YA之间)筛选出145个差异代谢物,其中上调代谢物为76种,下调为69种。代谢物主要集中在酚酸类、黄酮、有机酸、木脂素和香豆素、氨基酸及其衍生物和核苷酸及其衍生物等。KEGG代谢通路富集分析显示,这些差异代谢物主要富集在异黄酮生物合成代谢通路上。铝胁迫下,花生幼苗根系代谢物发生的明显改变,可为花生生产有效降低铝毒害提供依据。
-
-
吴家怡;
袁芳;
孟丽姣;
李晨阳;
史红松;
白岩松;
武晓如;
李加纳;
周清元;
崔翠
-
-
摘要:
随着土壤酸化的加剧,土壤中的Al^(3+)已成为限制光合作用、影响作物生长和产量的主要因素之一。本文以2400μmol L^(-1)铝溶液处理由甘蓝型油菜品种中双11号及10D130构建的高世代重组自交系(RIL)群体为研究对象,测定油菜铝毒胁迫下油菜苗期光合能力和叶绿素荧光相关性状,计算其相对值(处理/对照)并定位其QTL,根据QTL置信区间筛选合适的候选基因。研究结果表明,光合能力性状间和叶绿素荧光参数间分别都存在着显著相关,但是光合能力参数和叶绿素荧光参数之间相关性不显著;除相对T_(r)外,其余10个性状(相对P_(n)、相对G_(s)、相对C_(i)、相对Φ_(PSII)、相对F_(v)/F_(o)、相对F_(v')/F_(m')、相对q_(P)、相对ETR、相对NPQ和相对干重)共检测到16个与铝胁迫和光合相关性状QTL位点,分别位于A03、A05、A06、A08、A09、A10、C02和C09染色体上, LOD值介于2.05~3.18,能够解释的表型变异为8.1%~12.3%,其中在A03染色体106.038~109.129 cM位置上,相对G_(s)与相对F_(v')/F_(m')的位点完全重合, A06染色体上145.355~167.417 cM处检测到相对Φ_(PSII)和相对ETR的QTL置信区间完全重合。筛选到47个可能与抗铝胁迫及光合相关的候选基因,涉及到有机酸分泌、金属离子转运、激素调节、启动抗氧化保护以及叶绿体自身调节、磷元素摄入调节等方面。本研究为油菜铝抗性及相关光合基因功能挖掘和油菜耐铝高光合品种选育奠定基础。
-
-
汤霞;
陈仕勇;
周青平
-
-
摘要:
为研究铝胁迫对燕麦种子萌发及幼苗生长的影响,本试验以5个燕麦品种(“莫妮卡”“梦龙”甜燕2号“牧王”“摄政王”)为材料,在酸性环境下(pH=4.5)进行不同浓度Al^(3+)(0、2.5、5、10、20 mmol/L)胁迫处理。结果表明,Al^(3+)胁迫处理对5个燕麦品种的萌发未产生较大影响,而对其幼苗的株高、根长、根干重、根鲜重、茎叶干重、茎叶鲜重等均有不同程度的抑制作用,且对根的抑制程度大于茎叶(p“牧王”>“莫妮卡”>“梦龙”>甜燕2号。
-
-
罗涵夫;
宋华伟;
刘天增;
张巨明
- 《中国草学会草坪专业委员会第十五次学术研讨会》
| 2016年
-
摘要:
镧是稀土元素中最重要和最活泼的元素,具有促进种子萌发、提高作物产量和增强抵御逆境胁迫等作用.以华南地区水土保持常用的百喜草的幼苗为研究对象,将人工萌发处理7天后的百喜草幼苗,在培养液中含有0.9μM A13+的酸性铝胁迫条件下水培,处理组在0.9μMAl3+的酸性铝胁迫条件下加入0.3μM的La3+水培.在水培的第2、4、6天,分离幼苗的根与茎,分别测定根系形态、根干鲜比、根茎比、根系活力,探究在酸性铝胁迫条件下氯化镧对百喜草幼苗根生长的影响.结果表明:所有测定时段Al+La处理的百喜草幼苗的主根长、根表面积、根尖数、根体积、根干鲜比、根茎比、根系活力均高于A1处理;第6d的测定中,Al+La处理的总根长、根尖数、根表面积和根体积显著高于A1处理;第2、4d测定中,Al+La处理的根系活力都显著高于A1处理.说明在酸性铝胁迫条件下,镧元素处理可以促进百喜草幼苗根系的生长.
-
-
张美艳;
薛世明;
钟声;
张英俊;
黄必志
- 《第三届(2014)中国草业大会》
| 2014年
-
摘要:
实验选取我国西南地区的几个主栽鸭茅品种德纳塔(Donata)、安巴(Amba)、宝兴(Baoxing)和牧友(Potomac)为研究对象,设计了酸性环境下(pH4.5)不同梯度的铝离子处理(0、10、30、50和100uM)实验.研究鸭茅植株生长及形态对铝胁迫的耐受响应.结果表明,铝胁迫下,鸭茅枝条干重、根系干重、植株形态及根系伸长均受到显著的抑制作用,且随着浓度的增加呈现出下降趋势;低浓度(10~30uM)铝离子处理对鸭茅生长造成的抑制较弱,在一定程度上有利于植株形成根冠较为稳定的形态,而高浓度(≧50uM)铝离子处理则对鸭茅植株根系伸长和根系形态参数造成严重的抑制,不利于植株后期的健康生长;供试鸭茅枝条和根系积累铝的耐受响应不一致,根系积累铝的数量要远远大于枝条;且品种间存在一定差异,牧友根系中铝含量最低,耐受性较强.研究得出,鸭茅通过改变植株形态和调控自身积累铝的水平来应对铝胁迫,但是酸性土壤上的铝离子对鸭茅的植株生长及形态塑造仍造成一定程度的影响.
-
-
-
刘颖坤;
桂仁意;
喻卫武;
方伟
- 《中国林学会竹子分会四届四次全委会暨第六届中国竹业学术大会》
| 2010年
-
摘要:
以毛竹种子作为实验材料,建立植物根系分泌物中有机酸的提取和测定方法,并测定铝胁迫下毛竹根系分泌物中有机酸.以毛竹水培法收集根系分泌物,分别以0,100,500,1000,2000 μmol/L5种不同浓度AlCl3溶液作为培养液进行培养,旋转蒸发浓缩、定容;使用ACQUITY TM Ultra Performance LC超高效液相色谱系统,以3%的CH3OH-K2HPO4 (0.01 mol/L) (pH 2.6) ( V/ V)溶液作流动相,经HSS T3 C18柱分离,考察检测波长、色谱柱、流动相的pH值及分离温度等因素对4种有机酸分离效果的影响.结果表明,本方法样品制备简便,分离效果好,方法的线性范围及精密度、准确度和回收率都能满足毛竹根系分泌物中痕量有机酸定性和定量分析的要求.根据分析物的保留时间以及光谱图对样品色谱峰定性,水培毛竹根系分泌物中可以检出的有机酸为草酸和苹果酸;铝离子的存在对毛竹根系有机酸的分泌有促进作用,500 μmol/L的AlCl3溶液对有机酸分泌的促进作用最强.
-
-
-
邱晓;
张兴兴;
闫雯;
安渊
- 《中国草学会青年工作委员会学术研讨会暨草业科技创新与草业发展大会》
| 2010年
-
摘要:
在pH4.5和100μmol铝离子浓度环境条件下,采用水培方法对27个紫花菖蓿品种的铝敏感程度进行评价。通过分析铝胁迫处理植株的根长、株高、茎叶生物量和根系生物量的相对生长量,以及根系活力、丙二醛含量和Fv/Fm值的变化,并利用聚类分析方法对27个紫花苜蓿品种进行了耐铝性能的聚类,结果表明,27个品种的耐铝性能存在很大差异,依据其耐铝性能的差异,将27个紫花苜蓿品种分成四类,其中,第Ⅳ类的四个品种:巨人802、丰宝、WL525和萨兰多的铝敏感性最低,耐铝性能最强,该类组的根长、茎叶生物量、根系生物量和根系活力的相对抑制牢分别比敏感类(第1类)低18.43%、34.44%、38.59%,81.65%,本研究结果对选择在酸性土壤种植的紫花苜蓿品种具有很好的指导作用。
-
-
- 《2007年广东省植物学会青年学术研讨会暨第二届广东省植物学研究生学术研讨会》
| 2007年
-
摘要:
Aluminum(Al)toxicity is a serious limitation to worldwide crop production.Rice is one of the most Al-tolerant crops and also serves as an important monocot model plant.This study aims to identify Al-responsive proteins in flee,based on evidence that Al resistance is an inducible process.Two Al treatment systems were applied in the study:Al3+-containing simple Ca solution culture and Al3+-containing complete nutrient solution culture.Proteins prepared from rice roots were separated by 2-DE.The 2-DE patterns were compared and the differentially expressed proteins were identified by MS.A total of 17 Al-responsive proteins were identified,with 12 of which being up-regulated and 5 down-regulated.Among the up-regulated proteins are Cu-Zn SOD,GST and S-adenosylmethionine synthetase 2,which are the consistently known Al-induced enzymes previously detected at the transcriptional level in other plants.More importantly,a number of other identified proteins including cysteine synthase(CS),1-aminecyclopropane-1-carboxylate oxidase,G protein β subunit-like protein,ABA-and stress-induced protein,putative Avr9/Cf-9 rapidly elicited protein 141 and 33 kDa secretory protein are novel Al-induced proteins.Most of these proteins are functionally associated with signaling transduction,antioxidation and detoxification.CS,as consistently detected in both Al stress systems,was further validated by Western blot and CS activity assays.Moreover,the metabolic products of CS catalysis,I.E.Both the total glutathione pool and reduced glutathione,were also significantly increased in response to Al stress.Taken together,our results suggest that antioxidation and detoxification ultimately related to sulfur metabolism,particularly to CS,mayplayafunctional role in Al adaptation for rice.
-
-
- 《2007年广东省植物学会青年学术研讨会暨第二届广东省植物学研究生学术研讨会》
| 2007年
-
摘要:
Aluminum(Al)toxicity is a serious limitation to worldwide crop production.Rice is one of the most Al-tolerant crops and also serves as an important monocot model plant.This study aims to identify Al-responsive proteins in flee,based on evidence that Al resistance is an inducible process.Two Al treatment systems were applied in the study:Al3+-containing simple Ca solution culture and Al3+-containing complete nutrient solution culture.Proteins prepared from rice roots were separated by 2-DE.The 2-DE patterns were compared and the differentially expressed proteins were identified by MS.A total of 17 Al-responsive proteins were identified,with 12 of which being up-regulated and 5 down-regulated.Among the up-regulated proteins are Cu-Zn SOD,GST and S-adenosylmethionine synthetase 2,which are the consistently known Al-induced enzymes previously detected at the transcriptional level in other plants.More importantly,a number of other identified proteins including cysteine synthase(CS),1-aminecyclopropane-1-carboxylate oxidase,G protein β subunit-like protein,ABA-and stress-induced protein,putative Avr9/Cf-9 rapidly elicited protein 141 and 33 kDa secretory protein are novel Al-induced proteins.Most of these proteins are functionally associated with signaling transduction,antioxidation and detoxification.CS,as consistently detected in both Al stress systems,was further validated by Western blot and CS activity assays.Moreover,the metabolic products of CS catalysis,I.E.Both the total glutathione pool and reduced glutathione,were also significantly increased in response to Al stress.Taken together,our results suggest that antioxidation and detoxification ultimately related to sulfur metabolism,particularly to CS,mayplayafunctional role in Al adaptation for rice.
-
-
- 《2007年广东省植物学会青年学术研讨会暨第二届广东省植物学研究生学术研讨会》
| 2007年
-
摘要:
Aluminum(Al)toxicity is a serious limitation to worldwide crop production.Rice is one of the most Al-tolerant crops and also serves as an important monocot model plant.This study aims to identify Al-responsive proteins in flee,based on evidence that Al resistance is an inducible process.Two Al treatment systems were applied in the study:Al3+-containing simple Ca solution culture and Al3+-containing complete nutrient solution culture.Proteins prepared from rice roots were separated by 2-DE.The 2-DE patterns were compared and the differentially expressed proteins were identified by MS.A total of 17 Al-responsive proteins were identified,with 12 of which being up-regulated and 5 down-regulated.Among the up-regulated proteins are Cu-Zn SOD,GST and S-adenosylmethionine synthetase 2,which are the consistently known Al-induced enzymes previously detected at the transcriptional level in other plants.More importantly,a number of other identified proteins including cysteine synthase(CS),1-aminecyclopropane-1-carboxylate oxidase,G protein β subunit-like protein,ABA-and stress-induced protein,putative Avr9/Cf-9 rapidly elicited protein 141 and 33 kDa secretory protein are novel Al-induced proteins.Most of these proteins are functionally associated with signaling transduction,antioxidation and detoxification.CS,as consistently detected in both Al stress systems,was further validated by Western blot and CS activity assays.Moreover,the metabolic products of CS catalysis,I.E.Both the total glutathione pool and reduced glutathione,were also significantly increased in response to Al stress.Taken together,our results suggest that antioxidation and detoxification ultimately related to sulfur metabolism,particularly to CS,mayplayafunctional role in Al adaptation for rice.
-
-
- 《2007年广东省植物学会青年学术研讨会暨第二届广东省植物学研究生学术研讨会》
| 2007年
-
摘要:
Aluminum(Al)toxicity is a serious limitation to worldwide crop production.Rice is one of the most Al-tolerant crops and also serves as an important monocot model plant.This study aims to identify Al-responsive proteins in flee,based on evidence that Al resistance is an inducible process.Two Al treatment systems were applied in the study:Al3+-containing simple Ca solution culture and Al3+-containing complete nutrient solution culture.Proteins prepared from rice roots were separated by 2-DE.The 2-DE patterns were compared and the differentially expressed proteins were identified by MS.A total of 17 Al-responsive proteins were identified,with 12 of which being up-regulated and 5 down-regulated.Among the up-regulated proteins are Cu-Zn SOD,GST and S-adenosylmethionine synthetase 2,which are the consistently known Al-induced enzymes previously detected at the transcriptional level in other plants.More importantly,a number of other identified proteins including cysteine synthase(CS),1-aminecyclopropane-1-carboxylate oxidase,G protein β subunit-like protein,ABA-and stress-induced protein,putative Avr9/Cf-9 rapidly elicited protein 141 and 33 kDa secretory protein are novel Al-induced proteins.Most of these proteins are functionally associated with signaling transduction,antioxidation and detoxification.CS,as consistently detected in both Al stress systems,was further validated by Western blot and CS activity assays.Moreover,the metabolic products of CS catalysis,I.E.Both the total glutathione pool and reduced glutathione,were also significantly increased in response to Al stress.Taken together,our results suggest that antioxidation and detoxification ultimately related to sulfur metabolism,particularly to CS,mayplayafunctional role in Al adaptation for rice.
-
-
-
- 昆明理工大学
- 公开公告日期:2020.01.10
-
摘要:
本发明公开了
-
-
-
-
-
-
-
-