2种菊苣再生体系及遗传转化效率的比较

摘要

Effects of culture medium composition on callus induction, shoot regeneration and root formation from cotyledon explants in two kinds of chicory(Puna chicory and Commander chicory) were evaluated to optimize the plant regeneration system. The Aeluropus littoralis Na+/H+ antiporter (AINHX) gene, which encodes a Na+/H+ antiporter, was introduced into chicory to evaluate their genetic transformation efficiency. The results showed that the callus induction and shoot regeneration varied from chicory genotypes. The optimum medium for Puna chicory and Commander chicory were MS+1. 5 mg/L 6-BA + 0. 2 mg/L IBA and MS+1. 0 mg/L 6-BA + 0. 5 mg/L NAA,respectively. The optimum medium for Puna and Commander chicory root formation was 1/2MS+0. 1 mg/L NAA. The insertion of AINHX gene into chicory genome was confirmed by PCR. The genetic transformation efficiency of Puna chicory and Commander chicory were 10. 0% and 13. 3% respectively.%以普那菊苣和将军菊苣子叶为材料,通过植物组织培养的方法,探讨了不同激素浓度配比对二者愈伤组织诱导、芽分化以及根再生的影响,并通过农杆菌介导法将编码獐茅液泡膜Na+/H+逆向转运蛋白基因(AlNHX)导入菊苣中,比较普那菊苣和将军菊苣的遗传转化效率.结果表明:不同基因型的菊苣愈伤组织诱导和芽分化条件不同,普那菊苣最佳培养基为MS+1.5 mg/L6-BA+0.2 mg/L IBA;将军菊苣最佳培养基为MS+ 1.0 mg/L 6-BA+0.5 mg/L NAA;二者最佳生根培养基均为1/2MS+0.1mg/L NAA.获得的抗性芽经PCR检测,初步证实AINHX已插入到菊苣基因组中,且普那菊苣转化效率为10.0％,将军菊苣转化效率为13.3％.