外源钙和茉莉酸甲酯诱导番茄植株抗灰霉病研究

摘要

The disease index,reactive oxygen species(H2O2 and O2' )content and activities of CAT,SOD and POD were examined in tomato leaves treated with CaCl2 ,EGTA and methyl jasmonatcand then inoculated with Botrytis cinerea. Results showed that: ( 1 )Ca, MeJA and both combined treatment (MeJA + Ca) decreased the incidence rates of tomato seedlings by 32. 5% ,38. 0% and 54. 5% compared with control, while the treatment with MeJA + Ca caused a significant decrease of disease incidence by 32. 6% and 15. 3% as compared to the treatment with Ca or MeJ A. MeJA + EGTA treatment was higher by 30. 3% than that of MeJ A treatment, lower by 13. 1 % than that of EGTA treatment. The Ca handling was less by 34. 2% than that of EGTA treatment. (2)Compared with the control, Ca, MeJ A and MeJA + Ca treatment promoted the accumulation of reactive oxygen species in leaves. The active oxygen content of MeJA + Ca treatment was higher than that of single Ca or MeJA treatment. The accumulation of reactive oxygen species were inhibi-ted by MeJA+EGTA compared with MeJA,but higher than that of EGTA treatment. The active oxygen content of Ca treatment was higher than that of EGTA treatment. (3)The SOD,CAT,POD activities were increased significantly treated with Ca,MeJA and the highest was MeJA + Ca treatment. The treatment sprayed with MeJA + EGTA,however,reduced the antioxidant enzyme activities compared with MeJA,but enhanced compared with EGTA treatment. The antioxidant enzyme activities of Ca handling were higher than that of EGTA treatment. This suggested calcium could adjust resistance to B. Cinerea induced by methyl jasmonate. This result is related with the role that calcium promoted methyl jasmonate-induced accumulation of reactive oxygen and antioxidant enzyme activities.%以‘辽园多丽’番茄幼苗为材料,研究了经钙(Ca)、钙螯合剂(EGTA)和茉莉酸甲酯(MeJA)处理后接种番茄灰霉病幼苗叶片的病情指数、活性氧(H2O2、O2)含量和过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性的变化.结果显示:(1)Ca、MeJA、MeJA+Ca处理番茄幼苗的灰霉病发病率分别比对照显著降低32.5％、38.0％和54.5％,而MeJA+Ca处理又显著低于Ca、MeJA处理32.6％和15.3％；MeJA+ EGTA处理高于MeJA处理30.3％,但低于EGTA处理13.1％；Ca处理低于EGTA处理34.2％.(2)Ca、MeJA及MeJA+Ca处理番茄幼苗叶片中活性氧积累量高于对照,MeJA+Ca处理又高于Ca、MeJA处理；但MeJA+ EGTA处理活性氧积累量低于MeJA处理,而高于EGTA处理；Ca处理的活性氧含量高于EGTA处理.(3)Ca、MeJA及MeJA+Ca处理幼苗叶片的SOD、CAT、POD的活性均比对照提高,且以MeJA+Ca处理最高；而MeJA+ EGTA处理抗氧化酶活性低于MeJA处理,但高于EGTA处理；Ca处理抗氧化酶活性高于EGTA处理.研究表明,钙在茉莉酸甲酯诱导番茄抗灰霉病过程中具有重要调节作用,这种作用与钙促进茉莉酸甲酯诱导番茄活性氧积累和抗氧化酶活性有关.