东方百合‘演员’DXS基因的克隆与表达分析

摘要

以东方百合‘演员’花瓣为试验材料,采用RACE技术,克隆得到百合1脱氧木酮糖5-磷酸合成酶基因DXS的cDNA全长,命名为LeDXS(GenBank登录号为MF576067).序列分析表明,LeDXS基因cDNA序列全长2 471 bp,其中开放阅读框包含2 142 bp,编码713个氨基酸,相对分子量大小约为76.3 kD,等电点为6.65,化学式为C3370H5374N942O1016S32.系统进化分析结果显示,LeDXS与猕猴桃和万寿菊DXS聚为一类,属于Ⅰ型DXS,是功能较保守的一类.实时荧光定量PCR结果分析表明,LeDXS基因在不同品种百合花瓣中均有表达,且浓香型百合DXS基因表达量比淡香型和无香型百合高.该研究结果为百合DXS生物学功能研究奠定了基础,同时也为百合花香育种提供了理论依据.%The full-length eDNA sequence of the 1-deoxy-D-xylulose 5 phosphate synthase (DXS) gene from the petals of Lilium Oriental hybrid ‘Entertainer’ was cloned using RACE methods,named as LeDXS (GenBank accession number MF576067).The full length of LeDXS eDNA is 2 471 bp and it contains an open reading frame of 2 142 bp,encoding a putative protein of 713 amino acids.The predicted protein molecular weight and theoretical isoelectric point of LeDXS are 76.3 kD and 6.65,respectively.The formula of LeDXS is C3370H5374N942O1016S32.Phylogenetic analysis indicates that LeDXS,clustered with DXS of kiwi fruit and marigold proteins,belongs to the plant DXS Ⅰ cluster and is a functionally conserved protein.LeDXS gene was expressed in the petals of all cultivars based on qRT-PCR analysis.LeDXS had a higher expression level in strong-scented lilies compared to weak-scented and non-scented lilies.The results will benefit our biologically functional understanding of LeDXS,and further research of floral fragrance breeding of lilies.