普通小麦对仓储害虫玉米象的抗性QTL分析

摘要

Quantitative trait loci (QTLs) associated with Sitophilus zeamais resistance were identified using a population (127 re-combined inbred lines) from wheat cross Chuannong 16 X Chuanmai 42. In a stimulated storage environment, adult, larva, and egg of S. Zeamais were artificially fed with wheat seeds harvested in 2008 and 2009, respectively. Five QTLs located on chromosomes 3B, 2D, 3D, and 4B were detected with phenotypic contributions of 10.2%, 8.5%, 8.3%, 8.5%, and 11.3%, respectively. The QTLs located between Xbarc6 and Xgwmll2 on chromosome 3D were identified in seeds from both growing years, which explained 8.3-8.5% of the phenotypic variance. The positive allele on this locus was from parent Chuannong 16.%以普通小麦品种川农16与人工合成小麦衍生品种川麦42为亲本构建了含有127个株系的重组自交系(RIL)群体,将2008和2009年收获的种子用玉米象(Sitophilus zeamais)虫卵进行接种,并于2009年11月开始储藏,次年8月和10月进行虫害调查.利用复合区间作图法(CIM)对RIL群体的抗虫性进行QTL定位分析.在2008年收获群体中检测到位于3B、2D和3D染色体的3个QTL,贡献率依次为10.2％、8.5％和8.3％；在2009年收获群体中检测到位于3D和4B的2个QTL,贡献率分别为8.5％和11.3％.位于3D染色体Xbarc6-Xgwm 112标记区间内的QTL在年度间重复检测到,贡献率为8.3％～8.5％,抗性位点来自川农16.