为建立高粱种子纯度及真实性鉴定技术体系,构建高粱栽培品种DNA指纹图谱数据库是必要的。利用SSR荧光标记技术筛选出36对SSR荧光标记引物,构建我国高粱杂种优势利用以来中晚熟区主推的20个品种的SSR指纹图谱。36对SSR引物共扩增出235个等位基因,平均每个等位基因检测到多态性位点7个,多态性位点变化范围为2~12个。20个高粱品种36个SSR位点的遗传多样性指数和多态性信息量的变化范围分别为0.3490~0.8813和0.3144~0.8696,平均值分别为0.6976和0.6571。从36对SSR引物中筛选到多态性丰富的2对引物作为高粱品种鉴定的特征引物,2对SSR特征引物组合可区分所有供试品种。20个高粱品种的SSR指纹图谱互不相同,可以作为各品种特定的图谱,为品种鉴别提供依据。%To establish a standard technical system for purity and authenticity identification of sorghum seeds, it is necessary to construct a DNA fingerprinting database of major sorghum hybrids. The fluorescent marker detection of simple sequence repeat (SSR) was used to establish SSR fingerprints of 20 sorghum hybrids that are popularized in medium and late-maturing areas in China since heterosis has been utilized. Thirty-six pairs of SSR primers were screened and 235 alleles were amplified with the set of primers. The number of alleles per locus ranged from two to twelve with an average of seven. The range of gene diversity and polymorphism information content (PIC) at 36 SSR loci of 20 hybrids were 0.3490–0.8813 and 0.3144–0.8696, with an average of 0.6976 and 0.6571, respectively. Among 36 SSR primers, two primer pairs with high polymorphism were identified as the spe-cific primers for distinguishing the all 20 sorghum hybrids, resulting in the fingerprints for the 20 sorghum hybrids. The presented data and technology provides an important basis for hybrids identification in sorghum.
展开▼
