恩诺沙星对卤虫无节幼体的急性毒性

摘要

为明确恩诺沙星对卤虫无节幼体的急性毒性，设置了0，2，4，6，8，10，11，12，14 mg· mL-19个恩诺沙星浓度，分析24 h半最大效应浓度（24 h-EC50）、24 h半致死浓度（24 h-LC50）和48 h半最大效应浓度（48 h-EC50）、48 h半致死浓度（48 h-LC50）。结果表明，25℃条件下，24和48 h恩诺沙星对卤虫无节幼体的最大不致死浓度分别为0.4和0.2 mg· mL-1，使卤虫无节幼体全部死亡的最低浓度分别为1.4和1.1 mg· mL-1。采用概率单位法计算恩诺沙星对卤虫无节幼体的EC50和LC50。恩诺沙星对卤虫无节幼体24和48 h的EC50分别为（10.66±0.33）和（10.54±0.33） mg· mL-1，LC50分别为（9.46±0.33）和（7.71±0.33） mg· mL-1。结果表明，卤虫无节幼体对恩诺沙星的敏感性较低，常规使用范围内，对卤虫无节幼体的存活无明显影响。%A serial of concentration gradient of enrofloxacin were used to evaluate the effect of acute toxicity of enrofloxacin on Artemia nauplius.Nine different concentrations of enrofloxacin were tested in the study , which were 0, 2, 4, 6, 8, 10, 11, 12 and 14 mg· mL-1 .The 24 h-EC50 and 48 h-EC50 ( effective concentration for 50%reduction ) , and 24 h-LC50 and 48 h-LC50 ( lethal concentration for 50%) of enrofloxacin were calculated by the method of probability unit .The results showed that , at 24 h and 48 h, the maximum concentrations of enrofloxacin that could not make Artemia nauplius die were 0.4 mg· mL-1 and 0.2 mg· mL-1 , respectively .The minimum concentrations that made Artemia nauplius to die entirely were 1.4 mg· mL-1 and 1.1 mg· mL-1 , respectively .The 24 h-EC50 and 48 h-EC50 of enrofloxacin to Artemia nauplius were ( 10.66 ± 0.33 ) mg· mL-1 and ( 10.54 ±0.33 ) mg· mL-1 , respectively.The 24 h-LC50 and 48 h-LC50 of enrofloxacin to Artemia nauplius were (9.46 ±0.33) mg· mL-1 and (7.71 ±0.33) mg· mL-1, respectively.The results suggested that Artemia nauplius was not sensitive to enrofloxacin .