瓦松快繁体系的构建研究

摘要

Studies on the multiple shoots induction , propagation and rooting of Orostachys fimbriatus plantlet in vitro were conducted to construct a rapid propagation system for Orostachys fimbriatus.The results were as follows: the proliferation multiple of the stem was 11.69 when cultured with the MS+1.0mg· L-1 6-BA+0.2mg· L -1 IBA+20g · L -1 sucrose medium.Leaves inoculated on MS +1.5mg· L -1 6-BA+0.1mg· L-1 IBA+30g· L-1 sucrose medium could regenerate small adventitious buds well , and the number of regenerated bud per leaf was 9.71 .The vigorous proliferous shoots were inoculated on 1/2 MS+0.2mg· L-1 IBA+0.05mg· L-1 NAA medium for rooting, and the rooting rate was 100%, the root number was 12.3 per shoot .%以瓦松为研究材料，系统地研究了丛生芽的诱导、增殖及生根培养等，探索瓦松快繁的最佳途径并构建其快繁体系，结果表明：茎段接种于MS＋1.0 mg· L-16-BA＋0.2 mg· L-1 IBA＋20 g· L-1蔗糖培养基上，茎段增殖倍数可达11.69，叶片接种于MS＋1.5 mg· L-16-BA＋0.1 mg· L-1 IBA＋30 g· L-1蔗糖培养基上，能产生很好的不定芽，其再生芽数达9.71。在1/2 MS＋0.2 mg· L-1 IBA＋0.05 mg· L-1 NAA培养基中，生根率达100％，平均每株能产生12.3条根，植株生长健壮。