黑龙江茴鱼(Thymallus arcticus grubei Dybowski)的细胞遗传学分析

摘要

Metaphase chromosome specimens of Thymallus arcticus grubei Dybowsk injected with PHA and colchicin were prepared from short - term culture of kidney cells with air - drying technique. It' s diploid chromosome number was 2n = 92. The karyotype was composed of 36m, lOsm, 8st and 38t chromosomes, NF = 138. It's diploid nucleus DNA content was measured from the somatic cell of T. a. grubei using flow cytometer( Beckman cellLab Quant, made in USA) and the DNA content (2.3 pg/N) of erythrocytes of chick ( Callus sp) as standard. The T. a. grubei DNA content was 5.03 pg/N. According to the present results and reported date, T. a. grubei is possibly an tetraploid(4n).%采用体内注射PHA和秋水仙素,肾细胞短期培养,常规空气干燥法制备黑龙江茴鱼(Thymallus arcticus grubei Dybowski)的染色体.对其肾细胞染色体数目统计分析表明,黑龙江茴鱼染色体组有92条染色体,核型公式为2n=36m+10sm+8st+38t,其染色体总臂数(NF)为138.采用流式细胞分析仪,测定了黑龙江茴鱼的DNA含虽,与鸡血细胞标准对照相比为(1.44 4±0.07),以鸡红细胞DNA含量2.3 pg/N计,则黑龙江茴鱼的体细胞DNA含量为5.03 pg/N.与已知的报道比较,黑龙江茴鱼染色体数目和DNA含量都体现为四倍体特征.