明清板栗古树指纹图谱的构建

摘要

为实现对板栗古树资源的遗传鉴定和科学保护,利用SSR分子标记技术对分布较为集中的明清时期种植的33株板栗古树个体构建指纹图谱.选取13对SSR引物,采用荧光毛细管电泳技术对33株明清板栗古树进行多态检测,共检测出61个多态位点,平均每对引物的多态位点数为4.69个;多态性信息含量指数(PIC)变幅为0.0601～0.8009,平均为0.4103.依据13对SSR引物在33株板栗古树中扩增的特异带型组合,采用引物-带型组合法构建了33株板栗古树的指纹图谱.研究结果对板栗古树保护、种质鉴定、分类和育种具有重要的应用价值.%For the purpose of genetic identification and scientific protection of ancient chestnut trees,SSR technology was used to establish fingerprinting for 33 ancient chestnut trees,which were planted during Ming and Qing dynasties. The polymorphism of 33 ancient chestnut trees was analyzed with capillary electrophoresis detection method with fluorescent SSRs markers.Totally,61 polymorphic alleles were revealed by 13 SSR prim-er pairs,with an average of 4.69 for each primer pair.The polymorphism information content values of the prim-er pairs ranged from 0.0601 to 0.8009,with an average of 0.4103.A strategy of combining primer pair with distinct alleles for fingerprint construction was developed and applied to the 33 ancient chestnut trees.The pres-ent results provide valuable information for the protection, germplasm classification and identification, and breeding of chestnut.