中华蜜蜂工蜂监督及卵表面超微结构研究

摘要

To study the worker policing mechanism in Apis cerana cerana,queen-laid diploid eggs(QDG) and haploid eggs(QHG)and worker-laid haploid eggs(WHG)were introduced to worker and drone cells on the same comb in queen-right colony.In 2,5,12 and 24 h,the elimination rate of eggs were observed and cal-culated.The surface ultrastructure of queen-laid diploid and haploid eggs of Apis cerana cerana was compared with that of worker-laid eggs by SEM.The results showed that the elimination rate in 2,5,12 and 24 h was higher in worker-laid eggs than that in queen-laid diploid and haploid eggs(P<0.05),supporting the worker-policing hypothesis in Apis cerana cerana.In 2,5,12 and 24 h,no significant difference was found in the ultra-structure of the egg surface among QDG,QHG and WHG.%为研究中华蜜蜂(Apis cerana cerana)的工蜂监督机制,采用人工移取中华蜜蜂蜂王产的受精卵(QDG)、未受精卵(QHG)和工蜂产的未受精卵(WHG)至同一张巢脾的工蜂巢房和雄蜂巢房中,置于中华蜜蜂的有王群中,在2,5,12和24 h分别观察3种类型卵的清除率.同时利用电子扫描显微镜观察中华蜜蜂QDG、QHG和WHG三种类型卵在2,5,12和24 h的表面超微结构.结果表明:在2,5,12和24 h WHG的清除率都显著比QDG和QHG高(P<0.05),QDG和QHG之间均差异不显著(P>0.05),中华蜜蜂蜂群中存在工蜂监督现象;在2,5,12和24 h QDG、QHG和WHG 3种类型卵表面超微结构无显著差异.