香菇尿嘧啶营养缺陷型菌株的筛选与分子验证

摘要

筛选出对5’-FOA 敏感性较高的香菇‘L26’菌株，采用原生质体紫外诱变技术，获得‘L26’菌株18株尿嘧啶营养缺陷型突变菌株。分子验证表明：其中8株菌株的尿嘧啶合成代谢路径发生了基因突变。6个菌株的 pyrF 基因发生突变，2个菌株的 pyrG 基因发生突变。%The ‘L26’strain of L .edodes with higher sensitivity of 5 ’-FOA was selected,and 18 uracil auxotrophic mutant strains were obtained by UV mutagenesis of protoplast.Molecular verification showed that 8 mutant strains had genetic mutations in uracil synthetic metabolic pathways;there were pyrF gene mutations in 6 strains and pyrG gene mutations in 2 strains.