德国进境海棠树根际中厚皮拟毛刺线虫的鉴定

摘要

从上海口岸进境的德国海棠景观树根际分离到1种拟毛刺属线虫.雌虫双生殖腺对伸,受精囊无,精子分布于子宫内;阴门孔状,阴门骨化结构小,圆三角形到椭圆形,微分离.雄虫腹中颈乳突1个,位于排泄孔前;精子大,胞核香肠形;交合刺末端微腹弯,杆区有横纹;交合伞狭窄;泄殖腔前有3个腹中交配乳突.厚皮拟毛刺线虫特异引物UNIVERSAL-F/PACHYREV2-R检测为PCR阳性,其18S序列与GenBank中厚皮拟毛刺线虫的序列相似性为99.30％-100％,序列差异为0-4 bp.基于18S序列构建的系统发育树显示该种线虫与厚皮拟毛刺线虫位于同一聚类组内.经形态分类鉴定、PCR检测和序列分析,将此线虫鉴定为厚皮拟毛刺线虫Paratrichodorus pachydermus.这是我国口岸首次截获该种线虫.%One species of Paratrichodorus nematode was isolated from rhizosphere of cherry-apple tree imported from Germany at Shanghai Port.Female nematode was didelphic,lack of spermatheca.Its sperm was dispersed throughout the uterus and pore-like vulva rounded triangular or oval,with small vaginal sclerotized pieces.Male nematode had one ventromedian cervical papillae in front of excretory pore,large sperm cells with large sausage-shaped nucleus,distal end of spicule nearly straight,spicules shaft with striation,arrow bursa and three ventromedian precloacal supplements.PCR amplification was positive with specific primers for P.pachydermus.Sequence analysis revealed that its 18S-sequence had 99.30％-100％ identity with P.pachydermus in GenBank.Phylogenetic tree of the 18S sequences showed this species clustered with P.pachydermus.Based on the morphological characteristics,PCR detection and sequence analysis,this kind of nematode was identified as P.pachydermus.This is the first report of interception of P.pachydermus in China.