采用不连续聚丙烯酰胺凝胶电泳（PAEG）对麦芽四糖淀粉酶粗酶液进行分离纯化，经质谱分析确定了麦芽四糖酶条带，然后切胶回收获得高纯度的麦芽四糖酶。将获得的含有麦芽四糖酶的凝胶制成颗粒通过甲醛链接固定，获得固定化麦芽四糖酶。经薄层色谱和液相色谱分析发现：使用固定化酶生产的麦芽四糖纯度高于粗酶液，尤其是单糖杂质含量显著减少。此外，固定化麦芽四糖酶的比活力升高。%A discontinuous polyacrylamide gel electrophoresis (PAEG) was performed to isolate and purify the maltotetraose -producing amylase from their crude preparations .The bands containing amylase in the gel were detected by mass spectral analysis , and the bands containing high -purity maltotetraose -producing amylase were cut down from the gel .The gel containing maltote-traose-producing amylase was squeezed into small pellets , and then immersed in 10% formaldehyde for the linkage between gel and enzyme as well as the immobilization of the amylase , finally the immobilized maltotetraose -producing amylase was obtained .By the analysis of thin layer chromatography and high performance liquid chromatography , the maltotetraose produced by immobilized enzyme had higher purity than that did by the crude enzyme solution , especially it had a significant decrease in monosaccharide con-tent.In addition, our results also indicated that there was an increase in the specific activity of the immobilized maltotetraose -pro-ducing amylase.
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