Pilot Plant Production of Glucose with Glucoamylase Immobilized to Porous Silica.

摘要

Glucoamylase has been immobilized to porous silica and its kinetics and temperature stability determined in laboratory and pilot scale reactors. Reaction rates and stability were measured with acid,alpha-amylase,and acid-alpha-amylase thinned cornstarches. The immobilized glucoamylase was found to be very stable in both laboratory and pilot plant packed column reactors,with a half-life at 55degrees C under production conditions of 581hours. The enzyme in the pilot plant reactor lost approximately 25% of its initial activity after 90+days of operation at 38-40degrees C and over one year of storage at 4degrees C,mostly due to pore blockage by gelatinized starch due to incomplete starch thinning. The method of corn starch thinning to produce dextrin has a marked effect on final product distribution,glucose concentration,and dextrose equivalent (DE).