甘薯苗床镰刀菌病害分子检测及分析

摘要

从江苏、河南、河北等地育苗床上采集发病薯苗,利用组织分离法分离病原菌,通过形态学和分子生物学方法鉴定致病镰刀菌种类.共分离获得21株病原菌株,它们均产生两种孢子,大型孢子镰刀型,具3~5个分隔,小型孢子椭圆形或卵形,单胞或双胞.这些病原菌株的rDNA-ITS片段大小为544~568 bp,核苷酸一致率为46.56%~100%.系统进化树将供试病原菌分为3个组:组1为茄病镰刀菌组,包括本研究的9个分离物;组2为尖孢镰刀菌组,包括本研究的11个分离物;组3只包括2个分离物,即LN828191和本研究的分离物HNTY4.%The diseased sweet potato seedlings in seedbed were collected from Jiangsu,Henan and Hebei provinces,the pathogens were isolated by using tissue separation method,and the pathogenic Fusarium species were identified through using morphological and molecular biological methods.A total of 21 pathogenic strains were separated and identified,and they all produced two types of conidium: the macroconidium was falcate with 3~5 septa;the microconidium was oval with 0~1 septum.The fragment size of rDNA internal transcribed space (rDNA-ITS) in these pathogenic strains was 544~568 bp,and the nucleotide concordance rate among them was 46.56%~100%.These pathogenic strains were divided into 3 groups in phylogenetic trees: group 1 included 9 isolates in this study,which were identified as F.solani;group 2 included 11 isolates in this study,which were identified as F.oxysporum;group 3 included only 2 isolates,namely HNTY4 (F.oxysporum) in this study and LN828191.