水稻HGO基因启动子的克隆及转化鉴定

摘要

Gene HGO encodes 1,2-homogentisate dioxygenase, which functions in Tyr degradation pathway.In order to further investigate the expression characteristics of the HGO gene in rice, we constructed rice gene HGO promoter and GUS recombinant expression vector, and transformed this expression vector into Arabidopsis thaliana to obtain transgenic plants by using Agrobacterium-mediated floral dip method.By histochemical GUS staining, we found that the rice HGO gene promoter was highly expressed in the root, hypocotyl, petiole and cotyledon of A.thaliana, but it was lowly expressed in the euphylla vein and euphylla apex of A.thaliana.%HGO是编码酪氨酸降解途径中尿黑酸1,2双加氧酶的基因.探讨了水稻HGO基因的表达特征,构建了水稻HGO基因启动子与GUS表达载体,采用农杆菌介导的浸花法将该表达载体转入拟南芥中,获得转基因植株.通过GUS组织化学染色法检测,发现水稻HGO基因启动子在拟南芥根、下胚轴、叶柄和子叶中高度表达,在真叶中只有叶脉和叶尖上有微弱的表达.