近年来小麦孢囊线虫病在河北省发生十分普遍,给小麦生产造成了严重损失.可以侵染小麦的孢囊线虫种类很多,对于病原的准确鉴定是防治病害的基础.本试验从形态学和分子生物学两方面对从河北省不同地区采集到的37个小麦孢囊线虫群体进行了比较分析和鉴定.经过对线虫ITS-rDNA序列分析,发现采集到的线虫ITS区序列差异很小,为同一种类,其ITS-rDNA序列与燕麦孢囊线虫Heterodera avenae Wollenweber,1924的 ITS-rDNA 序列同源性高于99％.通过光学显微镜进行形态学测量和观察,其主要形态学特征与燕麦孢囊线虫 H.avenae 的相近.综合其形态与分子序列特征,确定河北省小麦孢囊线虫病的病原线虫为 H.avenae.%The cereal cyst nematode disease caused serious damage to the production of wheat in recent years. Accurate diagnosis is the basis for controlling this disease. 37 cereal cyst nematode populations were extracted from different areas of Hebei province in this study. The morphological and molecular characters of the nematodes were systemically studied. The difference of ITS-rDNA sequences of all the tested nematode was small and these se quences are less than 1 % differences between local populations and Heterodera avenae. The main morphological and morphometrical characteristics of different populations were studied. They were mostly close to H. Avenae in morphol ogy. We confirmed that the nematode populations that extracted from Hebei province are Heterodera avenae based on morphological, morphometrical and molecular characters.
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