花生黄曲霉抗性与糖原合成酶激酶-3的关系

摘要

前期研究证明糖原合成酶激酶(GSK3β)在花生黄曲霉敏感品种中上调表达.本研究对花生黄曲霉抗性品种和易感品种发育种子表达的GSK3进行了生物信息学和定量PCR的分析验证.结果表明,在抗性品系中,GSK3β基因在小果时期上调表达.由于GSK3β是油菜类甾醇信号传导的负调控因子,在细胞延长中起着抑制作用.因此推测GSK3β在花生果实发育中是种子和果实大小的抑制因子,与黄曲霉抗性有间接或直接的关系.%It has been reported that Glycogen synthase kinase-3 was upregulated in peanut developing seed coat of Aspergillus flavus sensitive line. In this paper, we analyszed the expression differences of GSK3β gene in developing seeds between Aspergillus flavus resistant lines and susceptible lines with bioinformatic methods and QPCR-methods. The results indicated in early fruits stage, GSK3β gene was upregulated in Aspergillus flavus resistant lines. GSK3β is negative regulator for brassinosteroids,which inhibits cell elongation. Thus it's possible that GSK3β is a inhibitative factor in sizes of seeds and fruits in peanut development, direct or indirect related to the resistance to Aspergillus flavus.