The objective of this study was to investigate the developmental changes of TR-β gene expression in different duck breeds during embryonic and neonatal period in pectorales and legs and their effects, on weight of pectorals and legs. Real time PCR was applied to compare the expression level of thyroid hormone receptor beta ( TR-β) mRNA in pectorales and legs of Gaoyou ducks and Jinding ducks at the 17,21 ,25,27 embryonic day and the age of 7 d. The results showed that,the profiles of TR-β mRNA of muscle in two duck breeds were consistent during early development,but there are some different in pectorales and legs. The trend of TR-β mRNA is from low to high in pectorales and legs during embryonic,the highest level is in 27 embryonic day. After hatch,the level of TR-β mRNA is decline in pectorals (P <0. 01) ,but keep high level in legs( P >0. 05 ). These results indicate that the expression of TR-β gene was significantly affected by age and organization, but not affected by breed. During embryonic and neonatal period of ducks,the expression of TRα mRNA in pectorals may play a positive regulatory role on the weight of muscles and embryo or body and the opposite regulatory role in legs. The correlation analysis showed that there was a strong positive linear correlation between the expression of TR-β mRNA and muscle weight in early development of duck. The results indicate that during embryonic and neonatal period of ducks,the expression of TR-β mRNA in pectorals may have a positive regulatory role on the weight of muscles.%为探讨TR-β基因在不同品种鸭胚胎期和出雏早期胸肌和腿肌中表达的发育性变化及其对胸腿肌重的影响.采用实时荧光定量PCR方法研究了高邮鸭和金定鸭胚胎期(17,21,25,27胚龄)和出雏早期(7日龄)胸腿肌中TR-βmRNA的表达水平.结果表明,TR-β mRNA在2个品种鸭肌肉早期发育中表现出一致的表达规律,但在腿肌和胸肌的发育性变化规律稍有不同:在胚胎期胸肌和腿肌中的TR-β mRNA表达量均呈先低后高的趋势,27胚龄时表达量最高；但出壳后胸肌中的TR-β mRNA表达量出现显著下降(P＜0.01),而腿肌仍维持高水平表达(P＞0.05).表明TR-β mRNA表达具有显著的胚(日)龄依赖性,并存在组织差异,但无品种差异.鸭发育早期胸腿肌中TR-β mRNA表达量与胸腿肌重的相关性分析表明,两者呈强的正相关,提示胸腿肌中TR-β mRNA的表达可能在鸭早期胸腿肌发育过程中发挥着正调控作用.
展开▼
