大豆公共遗传图谱C1连锁群SSR标记空白区段的填补

摘要

为进一步饱和大豆公共图谱SSR标记,以大豆育成品种冀豆12×地方品种ZDD03651组合的211个F6株系为作图群体,以Kosambi作图函数构建SSR标记遗传连锁图谱.结果表明,栽培大豆冀豆12与大豆地方品种ZDD03651间SSR标记多态率为44.6％,遗传图谱包含21个连锁群,117个SSR标记,遗传距离总长度1 501 cM,标记间平均距离15.6 cM,其中包含8个偏分离标记.与公共遗传图谱相比,位点间排列顺序、遗传距离和偏分离位点比例基本相同.将SSR新标记Barcsoyssr_4_1181、Barcsoyssr_4_1201、Barcsoyssr_4_1235和Barcsoyssr_5_1266整合到C1连锁群上,填补了国际大豆公共遗传图谱中C1连锁群94.62 ～120.12 cM之间的SSR标记空白区段.%Cross was made using bred varieties Jidou 12 × landrace soybean ZDD03651 ,get F6 RIL population with 211 single plants as the mapping population, construction of a SSR genetic linkage map with Kosambi mapping function, In order to saturate with SSR markers on public genetic map in the further. The polymorphic ratio was 44. 6% of SSR markers between Jidou 12 and ZDD03651. A total of 117 pairs of SSR markers on genetic linkage map,including 8 pairs distorted SSR markers,The resulting genetic linkage map covered 1 501 cM,with an average inter-marker distance of 15.6 cM, including 21 linkage groups. Compared with public genetic map, the order between points, genetic distance and the proportion of distorted markers basically the same. And eventually the development of new molecular markers Barcsoyssr_4_l 181 , Barcsoyssr_4_1201, Barcsoyssr_4_1235 , Barcsoyssr_5_1266 linked to the Cl linkage map,in order to complete the blank section on linkage group Cl between 94.62 cM and 120. 12 cM in the international.