牡丹反转录转座子RT基因的分离与序列分析

摘要

以牡丹基因组为模板,利用简并引物进行PCR扩增,得到了编码Ty1-copia类反转录转座子反转录酶(RT基因)的序列,测序后所得序列长度为240 bp,包含2个开放读码框,共编码63个氨基酸.经NCBI中BLAST比对结果显示,洛阳红牡丹RT基因与枸杞、甜菜等RT基因的同源性均在70％以上.经MEGA和DNAstar软件分析表明,该片段在进化上与梅、杨、茶和苹果等落叶木本植物的同源性序列存在更高的同源性和更近的亲缘关系,与枸杞、鹰嘴豆、番茄、草莓等草本植物的同源性序列亲缘性较远.%With degenerate primer,the Tyl-copio-like retrotransposon sequence fragments were amplified from peony (Paeonia suffruticosa Andrews. ) genomic DNA and sequemced, getting a 240-bp PCR fragment which contains two open reading frames and encodes 63 amino acids. The results of BLAST in CenBank showed that the RT gene sequence of peony had more than 70% similarity with those of Lycium chinense and Beta vulgaris ,etc. A phylo-genetic analysis by MEGA and DNAstar software showed that the sequence had higher homology and closer paternity with those of deciduous woody trees such as Prunus mume, Populus ciliate, Camellia sinensis and Malus x domestica than those of herbaceous plants such as L. barbarum ,Cicer arietinum,Lycopersicon esculenlum and Fragaria viridis.