来源和质量不同对山羊卵母细胞体外成熟率的影响

摘要

比较了卵母细胞级别对体外成熟率的影响,以及促卵泡素( FSH)处理山羊对卵母细胞采集的数量和质量的影响.结果表明:卵母细胞级别对体外成熟率有显著影响,不论是来源于FSH处理卵巢还是屠宰场卵巢,均是A、B级卵母细胞的成熟率高于C级卵母细胞,差异极显著(P＜0.01).但是A、B级卵母细胞之间差异不显著；卵巢来源对卵母细胞采集的数量和质量有显著影响,A、B级卵母细胞在获卵总数中所占的比例,平均每个卵巢获卵母细胞数及A、B级卵母细胞数,均是FSH处理卵巢高于屠宰场卵巢,差异显著(P＜0.05)；卵巢来源对卵母细胞体外成熟率有显著影响,来自于FSH处理卵巢的卵母细胞体外成熟率高于屠宰场卵巢(81.6％比67.6％),差异极显著(P＜0.01).%The aim of the present study was to compare the effects of oocytea grade on the rate of in vitro maturation , and the effect of the FSH-primed follicles on the numbers and quality of collecting oocytes. The result showed that; Oocytes grade had significant effects on the rate of in vitro maturation, grade A and B oocytes showed significantly higher percentage of maturation than that of Grade C .whatever the oocytes from the FSH-primed goats or from slaughthouse ovaries( P < 0. 01). There was no signficent difference between Grade A and B oocytes. Ovaries source had significant effects on the numbers and quality of collecting oocytes,FSH-primed ovaries was higher than slaught- ? House ovaries, whatever the the percentage of Grade A and B oocytes in the total ones( P < 0. 05) ,the numbers of total oocytes(P < 0. 05 ) and Grade A and B oocytes (P <0. 05 ) from each ovary. Ovaries source had significant effects on percentage of maturation, oocytes recovered from the FSH-primed goats showed significantly higher percentage of maturation than that of oocytes from slaughthouse ovaries ( P < 0.01) .