中国荷斯坦牛BoLA-DRB3基因的mRNA表达研究

摘要

为揭示牛MHC-DRB3上游调控区多态对其表达调控作用，利用PCR-SSCP和DNA序列测定技术分析并建立BoLA-DRB3倡exon2和BoLA-DRB3 URR之间的连锁关系。结合不同近端调控区与转录因子结合模式预测结果，选择3种不同上游调控序列的连锁组合，应用实时定量RT-PCR技术对牛MHC-DRB3基因mRNA相对表达量进行了验证分析。结果表明，上游调控区基因型为H17H17的BoLA-DRB3基因mRNA相对表达量显著高于（P＜0．01）基因型H1 H1及H5 H6。初步证明上游调控区中的多态会引起结构基因表达量的变化。%To study the regulation of upstream regulatory region polymorphisms on BoLA-DRB3 gene expres-sion,PCR-SSCP and DNA sequencing were used to identify the linkage relationship between BoLA-DRB3*exon2 and BoLA-DRB3 URR .Combined with the predicted results of binding mode between different URR and transcrip-tion factor,three linkage types were selected to detect the BoLA-DRB3 mRNA levels by relative Real-time PCR ex-periments .The results showed that the expression abundances of BoLA-DRB3 varied in different linkage types .The expression level of mRNA of BoLA-DRB3 gene linked with H17H17 was significant higher than H1H1 and H5H6 ( P<0 .01 ) .This preliminary proved that the polymorphism of upstream regulatory region can cause the expression change of BoLA-DRB3 structural gene .